| Detailed Information for Phage Melville |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Olivia Neilly and Conner Dunathan |
| Year Found | 2023 |
| Location Found | Boulder, CO United States |
| Finding Institution | University of Colorado Boulder |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 2°C |
| GPS Coordinates | 40.0 N, 105.0 W Map |
| Discovery Notes | Taken from soil next to a building after rainy/snowy conditions. A large amount of soil was collected that was near the sidewalk. |
| Naming Notes | We named our named Melville to ensure that we followed the phage naming rules. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 18, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1418 |
| Genome length (bp) | 43252 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 14 bases |
| Overhang Sequence | CCCCGCCGCAATGG |
| GC Content | 66.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | N |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Colorado Boulder |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are approximately 3 mm in diameter and have halo shapes around a majority of plaques. Morphology seen on the plaque assay supports the idea that temperate phages are present due to their cloudy appearance. |
| Morphotype | Siphoviridae |
| Number of Genes | 76 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PQ184816 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
178 |
| Pitt Freezer Box Grid# |
E4 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
