| Detailed Information for Phage Minima | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Dathan Stone |
| Year Found | 2017 |
| Location Found | Guilford, CT USA |
| Finding Institution | Southern Connecticut State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 41.3172 N, 72.7209 W Map |
| Discovery Notes | The sample was found in the morning, 70F and the soil was a mix of dry and wet. About 10mm was dug down, and grass was pulled to mix in with the soil sample. Since microbacterium foliorum was discovered in the phylosphere of grasses, I wanted to mix some of the grass into the soil. |
| Naming Notes | "Minima" is Latin for "tiny". Minima produces 1mm plaques that look like archery targets. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 2, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 9882 |
| Genome length (bp) | 17362 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CCCGCCCCA |
| GC Content | 68.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | EE |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewAlucard Anseraureola Aries55 Astro16 Avvas Azizam Aztec Baine BarBear Belthelas BinkBonk Bintii Biscayne Bodhi17 BonaeVitae BoomRoasted Bradley2 Bri160 BrokMonster Bullzi2019 Burgy BurtonThePup Cayde6 Cazares Charbie Chatham Cheesecake Ciel Clownery Concrete DaftyDuck Danimal Danno Dongwon Dooby Douggie EdElric Efeko Eightball Ekko14 Elspeth Erin Gabbspett Gardevoir Gillker Godfather GoldenBlanche Goobery HarperAnne Hernandez44 Horacetta Hulk Jahseh JaimeB Jannah Jerky JoBros JooneeDee Josuke JRok Kaijohn KayPaulus Kevanna KillerTomato Kojax4 KyriosFaba LaviMo Leafy LemonPepper LionelHutz Livingwater Loca Lola20 Lunatic Luxx Majesty ManAs Maneater MasterPitts McShie MelBee03 Miaurora Minima MirimarMandM MrGreen Naby Namsahir NeumannU Niffler Nobel Noelani Oaklynn Octopus Otwor Owens PaoPu Picnic Pondwater PoRanda Publix Puddles3 PuffyCat Quaker Rhogar Rhysand RoscoWindex Sage7 SantasSleigh Sara Scamander Scrunchy SeaWolves Sippinontea Slentz Slizam Snuffles Stem TeddyBoy TimoTea TinyTruffula TomBrady12 Trireme Upsilon Vanisius VitulaEligans Witch Wolfpack Yami Yasuo YertPhresh Yubaba |
| Annotating Institution | Southern Connecticut State University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque is 1mm, and has a small clear center, with a noticeable fuzzy ring around it. This resembles a bullseye target and is a temperate phage. |
| Morphotype | Siphoviridae |
| Number of Genes | 25 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewMinima_1 Minima_2 Minima_3 Minima_4 Minima_5 Minima_6 Minima_7 Minima_8 Minima_9 Minima_10 Minima_11 Minima_12 Minima_13 Minima_14 Minima_15 Minima_16 Minima_17 Minima_18 Minima_19 Minima_20 Minima_21 Minima_22 Minima_23 Minima_24 Minima_25 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH651181 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.7 x 10^10 |
| Pitt Freezer Box# | 72 |
| Pitt Freezer Box Grid# | B5 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |