Detailed Information for Phage NeumannU |
Discovery Information |
Isolation Host | Microbacterium foliorum NRRL B-24224 |
Found By | Matt Mastropaolo and Christina Zacconi |
Year Found | 2018 |
Location Found | Aston, PA USA |
Finding Institution | Neumann University |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | No |
Isolation Temperature | 30°C |
GPS Coordinates | 39.874167 N, 75.440889 W Map |
Discovery Notes | Found near the Thomas A. Bruder, Jr. Life Center on Neumann University's campus. In a small area of soil with no grass. |
Naming Notes | The phage is named for the school in which it was isolated and in tribute to the Sisters of Saint Francis our founding organization. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Aug 21, 2019 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina |
Approximate Shotgun Coverage | 6452 |
Genome length (bp) | 17445 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 9 bases |
Overhang Sequence | CCCGCCCCA |
GC Content | 68.7% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | EE |
Subcluster | -- |
Cluster Life Cycle | Lytic |
Other Cluster Members |
|
Annotating Institution | Neumann University |
Annotation Status | In GenBank |
Plaque Notes | Large clear plaques with diffuse edges. The plaques form in 24 hours. The plaques are approximately 3-4 mm in size. Phage Morphology: Head - 45.61 nm, Tail length - 105.26 nm, Tail width - 10.5 nm |
Morphotype | Siphoviridae |
Number of Genes | 25 |
Number of tRNAs | 0 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Annotation in DNA Master Format | Download |
Submitted Minimal DNA Master File | Download |
Author List | Download |
Annotation Cover Sheet | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MT657332 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
SEA Lysate Titer |
2.1*10^10 |
Pitt Freezer Box# |
86 |
Pitt Freezer Box Grid# |
H8 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
GenBank File for Phamerator | Download |