| Detailed Information for Phage NeumannU |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Matt Mastropaolo and Christina Zacconi |
| Year Found | 2018 |
| Location Found | Aston, PA USA |
| Finding Institution | Neumann University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 39.874167 N, 75.440889 W Map |
| Discovery Notes | Found near the Thomas A. Bruder, Jr. Life Center on Neumann University's campus. In a small area of soil with no grass. |
| Naming Notes | The phage is named for the school in which it was isolated and in tribute to the Sisters of Saint Francis our founding organization. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 21, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 6452 |
| Genome length (bp) | 17445 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CCCGCCCCA |
| GC Content | 68.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EE |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Neumann University |
| Annotation Status | In GenBank |
| Plaque Notes | Large clear plaques with diffuse edges. The plaques form in 24 hours. The plaques are approximately 3-4 mm in size.
Phage Morphology: Head - 45.61 nm, Tail length - 105.26 nm, Tail width - 10.5 nm |
| Morphotype | Siphoviridae |
| Number of Genes | 25 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT657332 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.1*10^10 |
| Pitt Freezer Box# |
86 |
| Pitt Freezer Box Grid# |
H8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
