| Detailed Information for Phage Sachet | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Florence Mayanga |
| Year Found | 2025 |
| Location Found | South Portland, ME USA |
| Finding Institution | Southern Maine Community College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 30°C |
| GPS Coordinates | 43.64716 N, 70.22783 W Map |
| Discovery Notes | The phage was isolated from garden soil near the broccoli plants in the SMCC garden. After enrichment with Microbacterium foliorum (host), plaque was observed on the first plate. The phage was then purified through five rounds of 6-plate dilutions. |
| Naming Notes | Sachet comes from french, which means bag. Named by Florence Mayanga during the SMCC phage discovery project. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 9, 2026 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 431 |
| Genome length (bp) | 17362 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CCCGCCCCA |
| GC Content | 68.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | EE |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Unknown at this stage, further testing required. |
| Other Cluster Members |
Click to ViewAlucard Anseraureola Aries55 Astro16 Avvas Azizam Aztec Baine BarBear Belthelas BinkBonk Bintii Biscayne Bodhi17 BonaeVitae BoomRoasted Bradley2 Bri160 BrokMonster Bullzi2019 Burgy BurtonThePup Cayde6 Cazares Charbie Chatham Cheesecake Ciel Clownery Concrete DaftyDuck Danimal Danno DirtPie Dongwon Dooby Douggie EdElric Efeko Eightball Ekko14 ELee24 Elspeth Erin Gabbspett Gardevoir Gillker Godfather GoldenBlanche Goobery Goulash Grayscar HarperAnne HeadMave Hernandez44 Horacetta Hulk IzzyPants24 Jahseh JaimeB Jannah Jella Jerky JoBros JooneeDee Josuke JRok Kaijohn KayPaulus Kevanna KillerTomato Kojax4 KyriosFaba LaviMo Leafy LemonPepper LionelHutz Livingwater Loca Lola20 Lunatic Luxx Madebyisha Majesty ManAs Maneater Marichuy MasterPitts MauiIvory McShie MelBee03 Miaurora Minima MirimarMandM MrGreen Myram Naby Namsahir NeumannU Niffler Nobel Noelani Oaklynn Octopus OdetoJoy Otwor Owens PaoPu Picconator Picnic Pondwater PoRanda Publix Puddles3 PuffyCat Quaker Rhogar Rhysand Rira RoscoWindex Sachet Sage7 SantasSleigh Sara Scamander Scrunchy SeaWolves Sippinontea Slentz Slizam Snuffles Stem TeddyBoy TimoTea TinyTruffula TomBrady12 Trackstar Trireme Tyrellus Upsilon Vanisius VitulaEligans Witch Wolfpack Yami Yasuo YertPhresh Yubaba Zahlia |
| Annotating Institution | Southern Maine Community College |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | Plaques were clear and round, showing zones where bacteria were killed by phage. Plaques appeared after 3 days of incubation at 30 degrees |
| Number of Genes | 25 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
