| Detailed Information for Phage SeaWolves | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Former names | Phinders |
| Found By | Chelsea Lachance, Adeline McDonald, Abigail Ackor |
| Year Found | 2019 |
| Location Found | South Portland, ME United States |
| Finding Institution | Southern Maine Community College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 43.6459 N, 70.2276 W Map |
| Discovery Notes | We looked in the back of our Biology & Science Center building. We looked for dirt that had possible phage. We stayed near the building in hopes of finding phage that had never been discovered. |
| Naming Notes | We were searching for Phage, which was a new term and lesson in our Biology class. We originally had the name PhagePhinders but the website doesn't allow the word "phage". |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 13, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 5744 |
| Genome length (bp) | 17362 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CCCGCCCCA |
| GC Content | 68.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | EE |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewAlucard Anseraureola Aries55 Astro16 Avvas Azizam Aztec Baine BarBear Belthelas BinkBonk Bintii Biscayne Bodhi17 BonaeVitae BoomRoasted Bradley2 Bri160 BrokMonster Bullzi2019 Burgy BurtonThePup Cayde6 Cazares Charbie Chatham Cheesecake Ciel Clownery Concrete DaftyDuck Danimal Danno Dongwon Dooby Douggie EdElric Efeko Eightball Ekko14 Elspeth Erin Gabbspett Gardevoir Gillker Godfather GoldenBlanche Goobery HarperAnne Hernandez44 Horacetta Hulk Jahseh JaimeB Jannah Jerky JoBros JooneeDee Josuke JRok Kaijohn KayPaulus Kevanna KillerTomato Kojax4 KyriosFaba LaviMo Leafy LemonPepper LionelHutz Livingwater Loca Lola20 Lunatic Luxx Majesty ManAs Maneater MasterPitts McShie MelBee03 Miaurora Minima MirimarMandM MrGreen Naby Namsahir NeumannU Niffler Nobel Noelani Oaklynn Octopus Otwor Owens PaoPu Picnic Pondwater PoRanda Publix Puddles3 PuffyCat Quaker Rhogar Rhysand RoscoWindex Sage7 SantasSleigh Sara Scamander Scrunchy SeaWolves Sippinontea Slentz Slizam Snuffles Stem TeddyBoy TimoTea TinyTruffula TomBrady12 Trireme Upsilon Vanisius VitulaEligans Witch Wolfpack Yami Yasuo YertPhresh Yubaba |
| Annotating Institution | Columbia State Community College |
| Annotation Status | In GenBank |
| Plaque Notes | When creating plaques, we made sure there was no contamination when moving from each level with dilutions. We needed to infect the culture tubes before creating the plaque assays and be careful when pouring the petri dish to cover and not have bubbles creating any confusion. After being incubated, we found phage after several tests without any lysate. |
| Number of Genes | 25 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewSeaWolves_1 SeaWolves_2 SeaWolves_3 SeaWolves_4 SeaWolves_5 SeaWolves_6 SeaWolves_7 SeaWolves_8 SeaWolves_9 SeaWolves_10 SeaWolves_11 SeaWolves_12 SeaWolves_13 SeaWolves_14 SeaWolves_15 SeaWolves_16 SeaWolves_17 SeaWolves_18 SeaWolves_19 SeaWolves_20 SeaWolves_21 SeaWolves_22 SeaWolves_23 SeaWolves_24 SeaWolves_25 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OQ709210 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| GenBank File for Phamerator | Download |