| Detailed Information for Phage Slentz |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Jonah Slentz |
| Year Found | 2018 |
| Location Found | Cullowhee, NC USA |
| Finding Institution | Western Carolina University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 35.310278 N, 83.18722 W Map |
| Discovery Notes | Found under the bridge to parking lot 19A for the freshmen parking lot at Western Carolina University. Collected from fish feces at the edge of the water. |
| Naming Notes | The name of the phage came from my last name. The Slentz name is extremely exclusive. Each name can only be used once. Because of this, and how seriously I take this, no one else under the Slentz family name may be named “Phage.” |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 9, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1025 |
| Genome length (bp) | 17445 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CCCGCCCCA |
| GC Content | 68.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EE |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Western Carolina University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques have a clear center ranging from 2 to 3 mm with a halo ranging another 2 mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 25 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN010755 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
9x10^8 PFU/mL |
| Pitt Freezer Box# |
81 |
| Pitt Freezer Box Grid# |
D2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
