| Detailed Information for Phage Smurph | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Caitlin Murphy |
| Year Found | 2018 |
| Location Found | Manchester, NH USA |
| Finding Institution | Lehigh University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 7°C |
| GPS Coordinates | 42.9425 N, 71.457778 W Map |
| Discovery Notes | Soil sample collected on January 20, 2018 in Manchester, New Hampshire at the Manchester Waste Water Treatment Plant approximately ten feet from the stage two treatment tanks. The soil was frozen at the time of collection and very damp from the layer of snow once covering it. The sample was also very coarse and rocky, dark in color, and contained a few strands of grass which were scraped up with the soil during collection. |
| Naming Notes | The small size of the plaques it forms along with a family nickname of "Smurph" inspired the nomenclature for this phage. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 7, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1600 |
| Genome length (bp) | 43700 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 13 bases |
| Overhang Sequence | CCCGCCGCAATGG |
| GC Content | 66.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | N |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to ViewAggie Andies BabeRuth Bosection6 Butters Carcharodon Charlie Chewbacca Duplicity Fulbright Gex Hanako Jamie19 Journey Kevin1 Magsby Melville MichelleMyBell Nenae Panchino Parmesanjohn PhancyPhin Philonius Phloss Phrann Pipsqueaks Purgamenstris Raymond7 Rebel Redi Rubeelu Schnauzer Scitech ShrimpFriedEgg Shweta Silvafighter Silvy SkinnyPete Smurph Snekmaggedon SpongeBob Tapioca Xeno Xerxes |
| Annotating Institution | Lehigh University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are circular and less than 1mm in size. They are also slightly turbid, but mostly clear and uniform. |
| Morphotype | Siphoviridae |
| Number of Genes | 72 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewSmurph_1 Smurph_2 Smurph_3 Smurph_4 Smurph_5 Smurph_6 Smurph_7 Smurph_8 Smurph_9 Smurph_10 Smurph_11 Smurph_12 Smurph_13 Smurph_14 Smurph_15 Smurph_16 Smurph_17 Smurph_18 Smurph_19 Smurph_20 Smurph_21 Smurph_22 Smurph_23 Smurph_24 Smurph_25 Smurph_26 Smurph_27 Smurph_28 Smurph_29 Smurph_30 Smurph_31 Smurph_32 Smurph_33 Smurph_34 Smurph_35 Smurph_36 Smurph_37 Smurph_38 Smurph_39 Smurph_40 Smurph_41 Smurph_42 Smurph_43 Smurph_44 Smurph_45 Smurph_46 Smurph_47 Smurph_48 Smurph_49 Smurph_50 Smurph_51 Smurph_52 Smurph_53 Smurph_54 Smurph_55 Smurph_56 Smurph_57 Smurph_58 Smurph_59 Smurph_60 Smurph_61 Smurph_62 Smurph_63 Smurph_64 Smurph_65 Smurph_66 Smurph_67 Smurph_68 Smurph_69 Smurph_70 Smurph_71 Smurph_72 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK524518 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 5E10 |
| Pitt Freezer Box# | 84 |
| Pitt Freezer Box Grid# | F10 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
