The Actinobacteriophage Database

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Detailed Information for Phage TimoTea
Discovery Information
Isolation Host	Microbacterium foliorum NRRL B-24224
Found By	Ashlee Raymond
Year Found	2018
Location Found	Keystone, FL USA
Finding Institution	University of South Florida
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Unknown
Isolation Temperature	Not entered
GPS Coordinates	28.1186 N, 82.5766 W Map
Discovery Notes	Found by an isolated tree near a pond in Keystone, Florida.
Naming Notes	named after Timothee Chalamet, my favorite actor!
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Nov 28, 2018
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Approximate Shotgun Coverage	2510
Genome length (bp)	17427
Character of genome ends	3' Sticky Overhang
Overhang Length	9 bases
Overhang Sequence	CCCGCCCCA
GC Content	68.7%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	EE
Subcluster	--
Cluster Life Cycle	Lytic
Other Cluster Members	Click to View Alucard Anseraureola Aries55 Astro16 Avvas Azizam Aztec Baine BarBear Belthelas BinkBonk Bintii Biscayne Bodhi17 BonaeVitae BoomRoasted Bradley2 Bri160 BrokMonster Bullzi2019 Burgy BurtonThePup Cayde6 Cazares Charbie Chatham Cheesecake Ciel Clownery Concrete DaftyDuck Danimal Danno Dongwon Dooby Douggie EdElric Efeko Eightball Ekko14 Elspeth Erin Gabbspett Gardevoir Gillker Godfather GoldenBlanche Goobery HarperAnne Hernandez44 Horacetta Hulk Jahseh JaimeB Jannah Jerky JoBros JooneeDee Josuke JRok Kaijohn KayPaulus Kevanna KillerTomato Kojax4 KyriosFaba LaviMo Leafy LemonPepper LionelHutz Livingwater Loca Lola20 Lunatic Luxx Majesty ManAs Maneater MasterPitts McShie MelBee03 Miaurora Minima MirimarMandM MrGreen Naby Namsahir NeumannU Niffler Nobel Noelani Oaklynn Octopus Otwor Owens PaoPu Picnic Pondwater PoRanda Publix Puddles3 PuffyCat Quaker Rhogar Rhysand RoscoWindex Sage7 SantasSleigh Sara Scamander Scrunchy SeaWolves Sippinontea Slentz Slizam Snuffles Stem TeddyBoy TimoTea TinyTruffula TomBrady12 Trackstar Trireme Upsilon Vanisius VitulaEligans Witch Wolfpack Yami Yasuo YertPhresh Yubaba
Annotating Institution	University of South Florida
Annotation Status	In GenBank
Number of Genes	25
Number of tRNAs	0
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View TimoTea_1 TimoTea_2 TimoTea_3 (terminase) TimoTea_4 (portal protein) TimoTea_5 (major capsid and protease fusion protein) TimoTea_6 (head-to-tail adaptor) TimoTea_7 (tail terminator) TimoTea_8 (major tail protein) TimoTea_9 TimoTea_10 (tail assembly chaperone) TimoTea_11 (tail assembly chaperone) TimoTea_12 (tape measure protein) TimoTea_13 (minor tail protein) TimoTea_14 (minor tail protein) TimoTea_15 (minor tail protein) TimoTea_16 TimoTea_17 (endolysin) TimoTea_18 TimoTea_19 TimoTea_20 (Lsr2-like DNA bridging protein) TimoTea_21 (helix-turn-helix DNA binding domain protein) TimoTea_22 (helix-turn-helix DNA binding domain protein) TimoTea_23 TimoTea_24 (helix-turn-helix DNA binding domain protein) TimoTea_25 (HNH endonuclease)
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MK524502
Refseq Number	None yet
Published in Paper	Jacobs-Sera et al 2020, PLoS ONE: Genomic diversity of bacteriophages infecting Microbacterium spp
Archiving Info
Archiving status	Archived
SEA Lysate Titer	2.5 x 10^10 pfu/ml
Pitt Freezer Box#	84
Pitt Freezer Box Grid#	D1
Available Files
GenBank File for Phamerator	Download

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