| Detailed Information for Phage TimoTea |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Ashlee Raymond |
| Year Found | 2018 |
| Location Found | Keystone, FL USA |
| Finding Institution | University of South Florida |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 28.1186 N, 82.5766 W Map |
| Discovery Notes | Found by an isolated tree near a pond in Keystone, Florida. |
| Naming Notes | named after Timothee Chalamet, my favorite actor! |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 28, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2510 |
| Genome length (bp) | 17427 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CCCGCCCCA |
| GC Content | 68.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EE |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of South Florida |
| Annotation Status | In GenBank |
| Number of Genes | 25 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK524502 |
| Refseq Number | None yet |
| Published in Paper | Jacobs-Sera et al 2020, PLoS ONE: Genomic diversity of bacteriophages infecting Microbacterium spp |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.5 x 10^10 pfu/ml |
| Pitt Freezer Box# |
84 |
| Pitt Freezer Box Grid# |
D1 |
| Available Files |
| GenBank File for Phamerator | Download |