| Detailed Information for Phage TomBrady12 |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Sabrina Colindres |
| Year Found | 2020 |
| Location Found | Tampa, FL USA |
| Finding Institution | Hillsborough Community College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 33°C |
| GPS Coordinates | 27.57695 N, 82.2964 W Map |
| Discovery Notes | It was found in soil near a lawn, specifically in the front yard lawn of a neighborhood home in West Tampa by Cherry Street and MacDill Street. The lawn was freshly cut and seemed to be cut about 2-3 days before. The sample depth was about 2-3 inches deep. |
| Naming Notes | I named it TomBrady12 because recently in my hometown, the Tampa Bay Bucs recruited a 6 Time Super Bowl Champ Tom Brady. I've been a fan of Tom Brady since he played for the Patriots and I mean have you looked at him?!?! |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 18, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 5357 |
| Genome length (bp) | 17153 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CCCGCCCCA |
| GC Content | 68.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EE |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Hillsborough Community College |
| Annotation Status | In GenBank |
| Plaque Notes | My plaque morphology is clear and small in size and is circular in shape. Additionally, it was about 0.5 mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 26 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP405130 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
119 |
| Pitt Freezer Box Grid# |
B2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
