| Detailed Information for Phage Alucard |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Jakob O'Neal |
| Year Found | 2021 |
| Location Found | South Portland, ME United States |
| Finding Institution | Southern Maine Community College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 43.647224 N, 70.227765 W Map |
| Discovery Notes | The sample the phage was found in was taken from Southern Maine Community College's community garden. The sample was moist, dark soil taken from a tomato patch. |
| Naming Notes | I had Halloween on the brain when I was thinking of a name for the phage. While it isn't a perfect comparison, how phages survive and replicate remind me of vampires. "Alucard" is "Dracula" spelled backwards. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 13, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 5143 |
| Genome length (bp) | 17363 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CCCGCCCCA |
| GC Content | 68.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EE |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Southern Maine Community College |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques are 3 mm in diameter on average. The plaques appear to have present but not starkly apparent halos, indicating that the phages are temperate. |
| Morphotype | Siphoviridae |
| Number of Genes | 25 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR521072 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
183 |
| Pitt Freezer Box Grid# |
E10 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |