| Detailed Information for Phage Charbie |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Former names | Marlie |
| Duplicates of this Phage | LongOnyx |
| Found By | Laura Altfeld |
| Year Found | 2022 |
| Location Found | Wesley Chapel, FL USA |
| Finding Institution | Saint Leo University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 28.249373 N, 82.25206 W Map |
| Discovery Notes | This phage was isolated from a soil sample taken from my backyard vegetable bed. The soil is amended with compost (kitchen/garden waste and composted cow manure). |
| Naming Notes | The phage looks like a ball on a rope, so it’s affectionately named after my two dogs, Charlie and Mila. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 18, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 5592 |
| Genome length (bp) | 17428 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CCCGCCCCA |
| GC Content | 68.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EE |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Saint Leo University |
| Annotation Status | In GenBank |
| Number of Genes | 25 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP978816 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
166 |
| Pitt Freezer Box Grid# |
G3 |
| Available Files |
| GenBank File for Phamerator | Download |