| Detailed Information for Phage Josuke |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Elizabeth Childers |
| Year Found | 2020 |
| Location Found | Pittsburgh, PA United States |
| Finding Institution | Miami University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.368832 N, 80.067503 W Map |
| Discovery Notes | The sample was taken by another student who dropped the course, but I conducted the lab work when in-person classes resumed. |
| Naming Notes | My phage is named after Josuke Higashikata, my favorite character from Jojo's Bizzare Adventure, created by Hirohiko Araki. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 21, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1024 |
| Genome length (bp) | 17453 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CCCGCCCCA |
| GC Content | 68.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EE |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Webster University |
| Annotation Status | In GenBank |
| Number of Genes | 25 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OK310504 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
119 |
| Pitt Freezer Box Grid# |
B5 |
| Available Files |
| EM Picture | Download |
| GenBank File for Phamerator | Download |