| Detailed Information for Phage Nenae |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Alexa Alhout |
| Year Found | 2016 |
| Location Found | Fairfield, NJ USA |
| Finding Institution | Lehigh University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 40.883739 N, 74.305992 W Map |
| Discovery Notes | The soil was collected in approximately 35 degrees weather. The soil was taken from a mint garden. |
| Naming Notes | I chose the name Nenae (Nau-naa)because that is how you say mint in arabic. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Oct 17, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 3222 |
| Genome length (bp) | 42597 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 13 bases |
| Overhang Sequence | CCCGCCGCCTTGG |
| GC Content | 66.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | N |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Lehigh University |
| Annotation Status | In GenBank |
| Plaque Notes | The phage is a siphoviridae. The head is approximately 70 nm in length and width. The tail length is 200 nm. |
| Morphotype | Siphoviridae |
| Number of Genes | 70 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK524520 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.3x10^10 pfu/ml |
| Date of SEA Lysate Titering |
Mar 31, 2016 |
| Pitt Freezer Box# |
35 |
| Pitt Freezer Box Grid# |
C2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |