| Detailed Information for Phage Nenae | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Alexa Alhout |
| Year Found | 2016 |
| Location Found | Fairfield, NJ USA |
| Finding Institution | Lehigh University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 40.883739 N, 74.305992 W Map |
| Discovery Notes | The soil was collected in approximately 35 degrees weather. The soil was taken from a mint garden. |
| Naming Notes | I chose the name Nenae (Nau-naa)because that is how you say mint in arabic. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Oct 17, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 3222 |
| Genome length (bp) | 42597 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 13 bases |
| Overhang Sequence | CCCGCCGCCTTGG |
| GC Content | 66.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | N |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to ViewAggie Andies BabeRuth Bosection6 Butters Carcharodon Charlie Chewbacca Duplicity EGUnicorn Fulbright Gex Hanako Jamie19 Journey Kevin1 Magsby Melville MichelleMyBell Nenae Panchino Parmesanjohn PhancyPhin Philonius Phloss Phrann Pipsqueaks Purgamenstris Raymond7 Rebel Redi Rubeelu Schnauzer Scitech ShrimpFriedEgg Shweta Silvafighter Silvy SkinnyPete Smurph Snekmaggedon SpongeBob Tapioca Tortoise12 Xeno Xerxes |
| Annotating Institution | Lehigh University |
| Annotation Status | In GenBank |
| Plaque Notes | The phage is a siphoviridae. The head is approximately 70 nm in length and width. The tail length is 200 nm. |
| Morphotype | Siphoviridae |
| Number of Genes | 70 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewNenae_1 Nenae_2 Nenae_3 Nenae_4 Nenae_5 Nenae_6 Nenae_7 Nenae_8 Nenae_9 Nenae_10 Nenae_11 Nenae_12 Nenae_13 Nenae_14 Nenae_15 Nenae_16 Nenae_17 Nenae_18 Nenae_19 Nenae_20 Nenae_21 Nenae_22 Nenae_23 Nenae_24 Nenae_25 Nenae_26 Nenae_27 Nenae_28 Nenae_29 Nenae_30 Nenae_31 Nenae_32 Nenae_33 Nenae_34 Nenae_35 Nenae_36 Nenae_37 Nenae_38 Nenae_39 Nenae_40 Nenae_41 Nenae_42 Nenae_43 Nenae_44 Nenae_45 Nenae_46 Nenae_47 Nenae_48 Nenae_49 Nenae_50 Nenae_51 Nenae_52 Nenae_53 Nenae_54 Nenae_55 Nenae_56 Nenae_57 Nenae_58 Nenae_59 Nenae_60 Nenae_61 Nenae_62 Nenae_63 Nenae_64 Nenae_65 Nenae_66 Nenae_67 Nenae_68 Nenae_69 Nenae_70 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK524520 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.3x10^10 pfu/ml |
| Date of SEA Lysate Titering | Mar 31, 2016 |
| Pitt Freezer Box# | 35 |
| Pitt Freezer Box Grid# | C2 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |