| Detailed Information for Phage Puddles3 | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Chelsea Clark |
| Year Found | 2023 |
| Location Found | Pittsburgh, PA United States of America |
| Finding Institution | Chatham University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 22°C |
| GPS Coordinates | 40.4491 N, 79.92519 W Map |
| Discovery Notes | The sample was found in a garden bed, next to a puddle on the campus of Chatham University. |
| Naming Notes | Was named Puddles3 due to the sample being found next to a puddle, and to being the third sample we tested. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 23, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2501 |
| Genome length (bp) | 17362 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CCCGCCCCA |
| GC Content | 68.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | EE |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewAlucard Anseraureola Aries55 Astro16 Avvas Azizam Aztec Baine BarBear Belthelas BinkBonk Bintii Biscayne Bodhi17 BonaeVitae BoomRoasted Bradley2 Bri160 BrokMonster Bullzi2019 Burgy BurtonThePup Cayde6 Cazares Charbie Chatham Cheesecake Ciel Clownery Concrete DaftyDuck Danimal Danno Dongwon Dooby Douggie EdElric Efeko Eightball Ekko14 Elspeth Erin Gabbspett Gardevoir Gillker Godfather GoldenBlanche Goobery HarperAnne Hernandez44 Horacetta Hulk Jahseh JaimeB Jannah Jerky JoBros JooneeDee Josuke JRok Kaijohn KayPaulus Kevanna KillerTomato Kojax4 KyriosFaba LaviMo Leafy LemonPepper LionelHutz Livingwater Loca Lola20 Lunatic Luxx Majesty ManAs Maneater MasterPitts McShie MelBee03 Miaurora Minima MirimarMandM MrGreen Naby Namsahir NeumannU Niffler Nobel Noelani Oaklynn Octopus Otwor Owens PaoPu Picnic Pondwater PoRanda Publix Puddles3 PuffyCat Quaker Rhogar Rhysand RoscoWindex Sage7 SantasSleigh Sara Scamander Scrunchy SeaWolves Sippinontea Slentz Slizam Snuffles Stem TeddyBoy TimoTea TinyTruffula TomBrady12 Trireme Upsilon Vanisius VitulaEligans Witch Wolfpack Yami Yasuo YertPhresh Yubaba |
| Annotating Institution | Chatham University |
| Annotation Status | Overdue (Expected completion by 5/1/2024) |
| Morphotype | Siphoviridae |
| Number of Genes | 25 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewPuddles3_1 Puddles3_2 Puddles3_3 Puddles3_4 Puddles3_5 Puddles3_6 Puddles3_7 Puddles3_8 Puddles3_9 Puddles3_10 Puddles3_11 Puddles3_12 Puddles3_13 — tRNA Puddles3_14 Puddles3_15 Puddles3_16 Puddles3_17 Puddles3_18 Puddles3_19 Puddles3_20 Puddles3_21 Puddles3_22 Puddles3_23 Puddles3_24 Puddles3_25 Puddles3_26 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |