| Detailed Information for Phage Eightball | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Alexis Rivera-Manning |
| Year Found | 2020 |
| Location Found | Chester, PA United States |
| Finding Institution | Neumann University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 39.841972 N, 75.389932 W Map |
| Discovery Notes | Found at 9:24 pm in 71 F weather. |
| Naming Notes | The phage grew at a fast rate since the beginning days of lab. In pool, your goal is to get your either stripped or solids balls in each pocket before the other person. Once in the pockets you can now hit the final ball or "eightball" in the pocket to win. Like pool my phages development was quickier than others and im waiting for the end game to come to hit my final ball in the pocket. This gave me the idea of calling the phage eightball. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 14, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 5973 |
| Genome length (bp) | 17439 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CCCGCCCCA |
| GC Content | 68.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | EE |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewAlucard Anseraureola Aries55 Astro16 Avvas Azizam Aztec Baine BarBear Belthelas BinkBonk Bintii Biscayne Bodhi17 BonaeVitae BoomRoasted Bradley2 Bri160 BrokMonster Bullzi2019 Burgy BurtonThePup Cayde6 Cazares Charbie Chatham Cheesecake Ciel Clownery Concrete DaftyDuck Danimal Danno Dongwon Dooby Douggie EdElric Efeko Eightball Ekko14 Elspeth Erin Gabbspett Gardevoir Gillker Godfather GoldenBlanche Goobery HarperAnne Hernandez44 Horacetta Hulk Jahseh JaimeB Jannah Jerky JoBros JooneeDee Josuke JRok Kaijohn KayPaulus Kevanna KillerTomato Kojax4 KyriosFaba LaviMo Leafy LemonPepper LionelHutz Livingwater Loca Lola20 Lunatic Luxx Majesty ManAs Maneater MasterPitts McShie MelBee03 Miaurora Minima MirimarMandM MrGreen Naby Namsahir NeumannU Niffler Nobel Noelani Oaklynn Octopus Otwor Owens PaoPu Picnic Pondwater PoRanda Publix Puddles3 PuffyCat Quaker Rhogar Rhysand RoscoWindex Sage7 SantasSleigh Sara Scamander Scrunchy SeaWolves Sippinontea Slentz Slizam Snuffles Stem TeddyBoy TimoTea TinyTruffula TomBrady12 Trireme Upsilon Vanisius VitulaEligans Witch Wolfpack Yami Yasuo YertPhresh Yubaba |
| Annotating Institution | Neumann University |
| Annotation Status | In GenBank |
| Plaque Notes | Large round clear plaques about 2 mm in size. |
| Morphotype | Siphoviridae |
| Number of Genes | 25 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewEightball_1 Eightball_2 Eightball_3 Eightball_4 Eightball_5 Eightball_6 Eightball_7 Eightball_8 Eightball_9 Eightball_10 Eightball_11 Eightball_12 Eightball_13 Eightball_14 Eightball_15 Eightball_16 Eightball_17 Eightball_18 Eightball_19 Eightball_20 Eightball_21 Eightball_22 Eightball_23 Eightball_24 Eightball_25 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OK040783 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.88 x 10^10 |
| Pitt Freezer Box# | 120 |
| Pitt Freezer Box Grid# | H11 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
