Microbacterium phage Goulash
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Detailed Information for Phage Goulash
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByRegan Leicht
Year Found2023
Location FoundAndover, SD Day
Finding InstitutionNorthern State University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature2°C
GPS Coordinates45.47049 N, 97.97829 W Map
Discovery NotesCollected on January 16, 2023 ~2 pm from feedlot where calves normally are. No cattle there when collected. Retrieved from about 2 inches below surface. Dry soil with little moisture, silty/sandy, some alfalfa pieces in sample plus loads of organic material. Sample was frozen and packed down; had to chisel out soil sample.
Naming NotesSo friggin' cold out that I came home and had a bowl of goulash to warm up. Thought that might be a good name.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 26, 2026
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Sequencer UsedIllumina NextSeq 1000
Read TypeSingle-end reads
Read Length100 bp
Approximate Shotgun Coverage5264
Genome length (bp)17362
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCCCGCCCCA
GC Content68.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEE
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionNorthern State University
Annotation StatusBeing Annotated (Expected completion by 5/1/2026)
Plaque Notes1-2 mm in diameter. Clear plaques occasionally fuzzy. Lytic most likely against M. foliorum.
Number of Genes25
Number of tRNAs1
Number of tmRNAs0
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives