Microbacterium phage Hernandez44
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Detailed Information for Phage Hernandez44
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByMelissa Kemp
Year Found2019
Location FoundPort St. Lucie, FL United States
Finding InstitutionIndian River State College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature28°C
GPS Coordinates27.2665 N, 80.319278 W Map
Discovery NotesCollected September 11th, 2019 at 12:37pm. The air temperature was 32.7°C. A large tree provided shade around the soil collection site. Patches of grass with scattered leaves were noted and a sidewalk was near, so apparent foot traffic was present. Soil was slightly moist. About an 1½ into the ground I collected about four tablespoons of the soil.
Naming NotesMy grandfather is my number one supporter, especially in education. If I ever feel defeated or not smart enough, he is always there to lift me up and remind me to never give up.Therefore, I decided to honor my grandfather with my first contribution to science. His name is Juan Hernandez and he was born in 1944.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 23, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage4015
Genome length (bp)17477
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCCCCGCCCCA
GC Content68.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEE
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionIndian River State College
Annotation StatusIn GenBank
Plaque NotesA bulls eye plaque with a cleared center and a turbid surrounding.
MorphotypeSiphoviridae
Number of Genes25
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT498046
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 98
Pitt Freezer Box Grid# A5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
GenBank File for PhameratorDownload