| Detailed Information for Phage JaimeB |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Keyshla G. Valentin Caban, India Wilkins |
| Year Found | 2022 |
| Location Found | Aston, PA United States |
| Finding Institution | Neumann University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 39.875331 N, 75.440021 W Map |
| Naming Notes | I named my phage after James Bond, initially I was struggling to have plaques; one day my plaques started to lyse a large quantity of plates. I came up with JaimeB, Phagent 007 Lysence to Kill. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 12, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 14635 |
| Genome length (bp) | 17445 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CCCGCCCCA |
| GC Content | 68.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EE |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Neumann University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques are clear with a round shape. The edges are slightly blurry, seen very minimally. |
| Number of Genes | 25 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR195050 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2x10^9 |
| Pitt Freezer Box# |
150 |
| Pitt Freezer Box Grid# |
G9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
