| Detailed Information for Phage Madebyisha |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Former names | mikeplaqueson |
| Duplicates of this Phage | ArcheaMcKenzie |
| Found By | Isha Drammeh and Curtis Wells |
| Year Found | 2024 |
| Location Found | Cumberland, MD United States |
| Finding Institution | Allegany College of Maryland |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 22°C |
| GPS Coordinates | 39.64953 N, 78.727002 W Map |
| Discovery Notes | The soil was collected at the Allegany College of Maryland Wetland. |
| Naming Notes | Named after one of the founders. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 9, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 8081 |
| Genome length (bp) | 17527 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CCCGCCCCA |
| GC Content | 68.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EE |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Allegany College of Maryland |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque appears lytic and transparent in plates. The plaques are also nine mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 25 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PV962793 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1 x 10^6 pfu/ml |
| Pitt Freezer Box# |
196 |
| Pitt Freezer Box Grid# |
C9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
