| Detailed Information for Phage Madebyisha | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Former names | mikeplaqueson |
| Duplicates of this Phage | ArcheaMcKenzie |
| Found By | Isha Drammeh and Curtis Wells |
| Year Found | 2024 |
| Location Found | Cumberland, MD United States |
| Finding Institution | Allegany College of Maryland |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 22°C |
| GPS Coordinates | 39.64953 N, 78.727002 W Map |
| Discovery Notes | The soil was collected at the Allegany College of Maryland Wetland. |
| Naming Notes | Named after one of the founders. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 9, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 8081 |
| Genome length (bp) | 17527 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CCCGCCCCA |
| GC Content | 68.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | EE |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewAlucard Anseraureola Aries55 Astro16 Avvas Azizam Aztec Baine BarBear Belthelas BinkBonk Bintii Biscayne Bodhi17 BonaeVitae BoomRoasted Bradley2 Bri160 BrokMonster Bullzi2019 Burgy BurtonThePup Cayde6 Cazares Charbie Chatham Cheesecake Ciel Clownery Concrete DaftyDuck Danimal Danno DirtPie Dongwon Dooby Douggie EdElric Efeko Eightball Ekko14 ELee24 Elspeth Erin Gabbspett Gardevoir Gillker Godfather GoldenBlanche Goobery HarperAnne HeadMave Hernandez44 Horacetta Hulk IzzyPants24 Jahseh JaimeB Jannah Jella Jerky JoBros JooneeDee Josuke JRok Kaijohn KayPaulus Kevanna KillerTomato Kojax4 KyriosFaba LaviMo Leafy LemonPepper LionelHutz Livingwater Loca Lola20 Lunatic Luxx Madebyisha Majesty ManAs Maneater MasterPitts McShie MelBee03 Miaurora Minima MirimarMandM MrGreen Myram Naby Namsahir NeumannU Niffler Nobel Noelani Oaklynn Octopus Otwor Owens PaoPu Picnic Pondwater PoRanda Publix Puddles3 PuffyCat Quaker Rhogar Rhysand RoscoWindex Sage7 SantasSleigh Sara Scamander Scrunchy SeaWolves Sippinontea Slentz Slizam Snuffles Stem TeddyBoy TimoTea TinyTruffula TomBrady12 Trackstar Trireme Upsilon Vanisius VitulaEligans Witch Wolfpack Yami Yasuo YertPhresh Yubaba Zahlia |
| Annotating Institution | Allegany College of Maryland |
| Annotation Status | Awaiting_QC |
| Plaque Notes | The plaque appears lytic and transparent in plates. The plaques are also nine mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 27 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewMadebyisha_1 Madebyisha_2 Madebyisha_3 Madebyisha_4 Madebyisha_5 Madebyisha_6 Madebyisha_7 Madebyisha_8 Madebyisha_9 Madebyisha_10 Madebyisha_11 Madebyisha_12 Madebyisha_13 Madebyisha_14 Madebyisha_15 — tRNA Madebyisha_16 Madebyisha_17 Madebyisha_18 Madebyisha_19 Madebyisha_20 Madebyisha_21 Madebyisha_22 Madebyisha_23 Madebyisha_24 Madebyisha_25 Madebyisha_26 Madebyisha_27 Madebyisha_28 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1 x 10^6 pfu/ml |
| Pitt Freezer Box# | 196 |
| Pitt Freezer Box Grid# | C9 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
