| Discovery Information |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | Coen McGarrah |
| Year Found | 2022 |
| Location Found | Tampa, FL United States |
| Finding Institution | University of South Florida |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 28.071111 N, 82.413056 W Map |
| Discovery Notes | Date Collected: 9/5/22
Conditions: 91° F, cloudy, 61% humidity
Location: Perimeter of Avalon Heights basketball courts
Notes: Dark, damp, slightly sandy, taken from about ½ an inch under the surface |
| Naming Notes | Named after the character Aziraphale from Good Omens by Neil Gaiman and Terry Pratchet. Aziraphale guarded the eastern gate of Eden with a flaming sword. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 28, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 777 |
| Genome length (bp) | 45336 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTAGGCAT |
| GC Content | 61.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CT |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of South Florida |
| Annotation Status | In GenBank |
| Plaque Notes | Small, turbid plaques with rough edges. Plaques grow slightly larger in higher concentrations. |
| Morphotype | Siphoviridae |
| Number of Genes | 67 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OQ709211 |
| Refseq Number | None yet |
| Published in Paper | McGarrah et al 2023, Microbiology Resource Announcements: Isolation and Annotation of Azira, a CT Cluster Phage That Infects Gordonia rubripertincta |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.86x10^11 PFU/mL |
| Pitt Freezer Box# |
149 |
| Pitt Freezer Box Grid# |
D11 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
