| Detailed Information for Phage Nibbles |
| Discovery Information |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | Jessica Chavez |
| Year Found | 2022 |
| Location Found | Las Vegas, NV US |
| Finding Institution | College of Southern Nevada |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.178972 N, 115.184189 W Map |
| Discovery Notes | Plaque was taken from a grass field from Lorenzi Park. This phage was taken from enriched isolation. |
| Naming Notes | This plaque is named after my older sister, whose nickname as a running joke is nibbles. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 9, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2696 |
| Genome length (bp) | 45601 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTAGGCAT |
| GC Content | 61.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CT |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | College of Southern Nevada |
| Annotation Status | In GenBank |
| Plaque Notes | Varying size morphology, varies from 1mm-2mm. Plaque formation seems concentration-dependent. The lower dilutions (with higher concentrations of phage) had fewer (and cloudier) plaques compared to the higher dilutions (with lower concentrations of phages when plating). Cloudy halo around perimeter of plaques. |
| Number of Genes | 67 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR253918 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
151 |
| Pitt Freezer Box Grid# |
H11 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
