Gordonia phage SweatNTears
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Detailed Information for Phage SweatNTears
Discovery Information
Isolation HostGordonia terrae 3612
Found ByJacob Cote, Ben Gove
Year Found2018
Location FoundME USA
Finding InstitutionUniversity of Maine, Honors College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates44.588706 N, 69.293424 W Map
Discovery NotesIsolated from soil that containing donkey manure compost. Sample taken from the Common Ground Fair in Unity, Maine.
Naming NotesThe first phage we isolated after many, many direct and enriched isolation attempts with other soil samples.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 15, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage637
Genome length (bp)45917
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGTAGGCTT
GC Content59.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCT
Subcluster--
Cluster Life CycleLytic
Lysogeny NotesEfficiency of lysogeny calculated to be 42.1% in G.terrae. Host range includes G. terrae, Rhodococcus erythropolis, and potentially Gordonia rubripertincta.
Other Cluster Members
Annotating InstitutionUniversity of Maine, Honors College
Annotation StatusIn GenBank
Plaque NotesSmall, pinpoint plaques with turbid halos around a fully cleared center. Variability in plaque morphology has been observed at high lysate concentrations.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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SweatNTears_1
(HNH endonuclease)

SweatNTears_2
(terminase small subunit)

SweatNTears_3

SweatNTears_4

SweatNTears_5

SweatNTears_6
(terminase large subunit)

SweatNTears_7
(portal protein)

SweatNTears_8
(capsid maturation protease)

SweatNTears_9
(scaffolding protein)

SweatNTears_10
(major capsid protein)

SweatNTears_11
(head-to-tail adaptor)

SweatNTears_12
(head-to-tail stopper)

SweatNTears_13

SweatNTears_14
(tail terminator)

SweatNTears_15
(major tail protein)

SweatNTears_16
(tail assembly chaperone)

SweatNTears_17
(tail assembly chaperone)

SweatNTears_18
(tape measure protein)

SweatNTears_19
(minor tail protein)

SweatNTears_20
(minor tail protein)

SweatNTears_21

SweatNTears_22
(lysin A, L-Ala-D-Glu peptidase domain)

SweatNTears_23
(lysin A, glycosyl hydrolase domain)

SweatNTears_24
(holin)

SweatNTears_25
(membrane protein)

SweatNTears_26

SweatNTears_27

SweatNTears_28

SweatNTears_29
(minor tail protein)

SweatNTears_30

SweatNTears_31

SweatNTears_32

SweatNTears_33

SweatNTears_34
(MerR-like helix-turn-helix DNA binding domain protein)

SweatNTears_35

SweatNTears_36

SweatNTears_37
(nuclease)

SweatNTears_38

SweatNTears_39

SweatNTears_40

SweatNTears_41
(RecA-like DNA recombinase)

SweatNTears_42
(Cas4 family exonuclease)

SweatNTears_43

SweatNTears_44
(DNA polymerase I)

SweatNTears_45
(nucleoside deaminase)

SweatNTears_46
(lysin B)

SweatNTears_47

SweatNTears_48
(thymidylate synthase)

SweatNTears_49

SweatNTears_50

SweatNTears_51

SweatNTears_52
(MazG-like nucleotide pyrophosphohydrolase)

SweatNTears_53
(kinase)

SweatNTears_54

SweatNTears_55
(DNA helicase)

SweatNTears_56

SweatNTears_57

SweatNTears_58

SweatNTears_59

SweatNTears_60
(DNA primase/helicase)

SweatNTears_61

SweatNTears_62

SweatNTears_63

SweatNTears_64

SweatNTears_65

SweatNTears_66

SweatNTears_67

SweatNTears_68

SweatNTears_69

SweatNTears_70

SweatNTears_71
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK967383
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 85
Pitt Freezer Box Grid# A5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload