| Detailed Information for Phage Button | |
| Discovery Information | |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | Zoe Aurelia Meyer |
| Year Found | 2021 |
| Location Found | Cullowhee, NC United States of America |
| Finding Institution | Western Carolina University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 16°C |
| GPS Coordinates | 35.3103 N, 83.1875 W Map |
| Discovery Notes | This Phage was found in the Cullowhee Creek on a rainy day under the bridge near the baseball fields. It was in a shallow part of the river. This sample is a water sample so the procedures to originally harvest it from the sample are a bit different than that of a soil sample. |
| Naming Notes | I named this phage after the nickname my Dad calls me. He is the one who inspired me to pursue and be passionate about the sciences. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 8, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 826 |
| Genome length (bp) | 46090 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTAGGCAT |
| GC Content | 62.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | CT |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewAgatha AikoCarson Amok AndPeggy Axym Azira BigChungus BillDoor Biskit Burnsey Button Buttrmlkdreams CherryonLim Cleo Cozz Dre3 Elinal Eliott Emalyn Feastonyeet Fribs8 Gibbous GiKK GoldHunter GTE2 Hexbug HippoPololi Horseradish Jamzy KayGee Lauer MAnor Margaret MaVan Mayweather MScarn MunkgeeRoachy Nibbles Nina Nodigi Orla Pons PotPie PsychoKiller Quasar RedBaron SheckWes SketchMex Sopespian SteamedHams SummitAcademy Survivors SweatNTears Tolls Troje Typhonomachy Vine Yakult Yarn Yummy Zareef |
| Annotating Institution | Western Carolina University |
| Annotation Status | In GenBank |
| Plaque Notes | This phage will plaque relatively small compared to other phages using a different bacterial host. Button also has a halo surrounding it most of the time, which can be noticeably seen on spot tests and spot titers. The phage also presents as having turbidity towards the edges of the plaque and clears up towards the most concentrated middle part. |
| Has been Phamerated? | Yes |
| Gene List |
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| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON970621 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 5x10^9 PFU/mL |
| Pitt Freezer Box# | 138 |
| Pitt Freezer Box Grid# | H7 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
