Gordonia phage Button
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Detailed Information for Phage Button
Discovery Information
Isolation HostGordonia rubripertincta NRRL B-16540
Found ByZoe Aurelia Meyer
Year Found2021
Location FoundCullowhee, NC United States of America
Finding InstitutionWestern Carolina University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature16°C
GPS Coordinates35.3103 N, 83.1875 W Map
Discovery NotesThis Phage was found in the Cullowhee Creek on a rainy day under the bridge near the baseball fields. It was in a shallow part of the river. This sample is a water sample so the procedures to originally harvest it from the sample are a bit different than that of a soil sample.
Naming NotesI named this phage after the nickname my Dad calls me. He is the one who inspired me to pursue and be passionate about the sciences.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 8, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage826
Genome length (bp)46090
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGTAGGCAT
GC Content62.7%
Fasta file available?Yes: Download fasta file
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionWestern Carolina University
Annotation StatusIn GenBank
Plaque NotesThis phage will plaque relatively small compared to other phages using a different bacterial host. Button also has a halo surrounding it most of the time, which can be noticeably seen on spot tests and spot titers. The phage also presents as having turbidity towards the edges of the plaque and clears up towards the most concentrated middle part.
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionON970621
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 5x10^9 PFU/mL
Pitt Freezer Box# 138
Pitt Freezer Box Grid# H7
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
GenBank File for PhameratorDownload