Gordonia phage PsychoKiller
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Detailed Information for Phage PsychoKiller
Discovery Information
Isolation HostGordonia rubripertincta NRRL B-16540
Found ByJon Mitchell
Year Found2020
Location FoundAberdeen, SD Brown
Finding InstitutionNorthern State University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature25°C
GPS Coordinates45.712853 N, 98.275606 W Map
Discovery NotesEnvironmental sample was taken from the wooded forest floor following a rain including several Ascomycete and Basidiomycete species attached to decaying/putrid branches/down trees. Luscious moss, sloppy soil, plant species, invertebrates, snails, bugs, rotten detritus. Very fertile soil sample in shade, small lighting between tree canopy. Birch, pine oak, maple, and ponderosa and white pine trees (needles and fallen/rotting leaves). Sample was harvested in September, 21 ~1:45 pm. Sample smells foul, actually beyond foul...very "juicy", "oozy" and "slimy". Quite dark. Failed to centrifuge distinctly and filtering with 0.22 micron slow and painful (use many filters and vacuum to recover ~10 microliters of sample.
Naming NotesNaming this virus I was listening to The Talking Heads song of the same name. This bacteriophage had a pretty high titer and was most reliable at lysing host.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 19, 2021
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2098
Genome length (bp)46085
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGTAGGCTT
GC Content60.2%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCT
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionNorthern State University
Annotation StatusBeing Annotated (Expected completion by 5/1/2021)
Plaque NotesLytic and round on Gordonia. 1-2 mm in diameter. Sometimes tiny. Sometime opaque also.
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives