| Detailed Information for Phage PsychoKiller | |
| Discovery Information | |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | Jon Mitchell |
| Year Found | 2020 |
| Location Found | Aberdeen, SD Brown |
| Finding Institution | Northern State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 25°C |
| GPS Coordinates | 45.712853 N, 98.275606 W Map |
| Discovery Notes | Environmental sample was taken from the wooded forest floor following a rain including several Ascomycete and Basidiomycete species attached to decaying/putrid branches/down trees. Luscious moss, sloppy soil, plant species, invertebrates, snails, bugs, rotten detritus. Very fertile soil sample in shade, small lighting between tree canopy. Birch, pine oak, maple, and ponderosa and white pine trees (needles and fallen/rotting leaves). Sample was harvested in September, 21 ~1:45 pm. Sample smells foul, actually beyond foul...very "juicy", "oozy" and "slimy". Quite dark. Failed to centrifuge distinctly and filtering with 0.22 micron slow and painful (use many filters and vacuum to recover ~10 microliters of sample. |
| Naming Notes | Naming this virus I was listening to The Talking Heads song of the same name. This bacteriophage had a pretty high titer and was most reliable at lysing host. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 19, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2098 |
| Genome length (bp) | 46085 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTAGGCTT |
| GC Content | 60.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | CT |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewAgatha AikoCarson Amok AndPeggy Axym Azira BigChungus Biskit Burnsey Button Buttrmlkdreams CherryonLim Cleo Cozz Dre3 Elinal Eliott Emalyn Feastonyeet Fribs8 Gibbous GiKK GTE2 Hexbug Horseradish Jamzy KayGee Lauer Margaret Mayweather MScarn Nibbles Nina Nodigi Orla Pons PsychoKiller Quasar SheckWes SketchMex Sopespian SteamedHams SummitAcademy Survivors SweatNTears Tolls Troje Vine Yakult Yarn Yummy |
| Annotating Institution | Northern State University |
| Annotation Status | Being Annotated (Expected completion by 5/1/2023) |
| Plaque Notes | Lytic and round on Gordonia. 1-2 mm in diameter. Sometimes tiny. Sometime opaque also. |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewPsychoKiller_Draft_1 PsychoKiller_Draft_2 PsychoKiller_Draft_3 PsychoKiller_Draft_4 PsychoKiller_Draft_5 PsychoKiller_Draft_6 PsychoKiller_Draft_7 PsychoKiller_Draft_8 PsychoKiller_Draft_9 PsychoKiller_Draft_10 PsychoKiller_Draft_11 PsychoKiller_Draft_12 PsychoKiller_Draft_13 PsychoKiller_Draft_14 PsychoKiller_Draft_15 PsychoKiller_Draft_16 PsychoKiller_Draft_17 PsychoKiller_Draft_18 PsychoKiller_Draft_19 PsychoKiller_Draft_20 PsychoKiller_Draft_21 PsychoKiller_Draft_22 PsychoKiller_Draft_23 PsychoKiller_Draft_24 PsychoKiller_Draft_25 PsychoKiller_Draft_26 PsychoKiller_Draft_27 PsychoKiller_Draft_28 PsychoKiller_Draft_29 PsychoKiller_Draft_30 PsychoKiller_Draft_31 PsychoKiller_Draft_32 PsychoKiller_Draft_33 PsychoKiller_Draft_34 PsychoKiller_Draft_35 PsychoKiller_Draft_36 PsychoKiller_Draft_37 PsychoKiller_Draft_38 PsychoKiller_Draft_39 PsychoKiller_Draft_40 PsychoKiller_Draft_41 PsychoKiller_Draft_42 PsychoKiller_Draft_43 PsychoKiller_Draft_44 PsychoKiller_Draft_45 PsychoKiller_Draft_46 PsychoKiller_Draft_47 PsychoKiller_Draft_48 PsychoKiller_Draft_49 PsychoKiller_Draft_50 PsychoKiller_Draft_51 PsychoKiller_Draft_52 PsychoKiller_Draft_53 PsychoKiller_Draft_54 PsychoKiller_Draft_55 PsychoKiller_Draft_56 PsychoKiller_Draft_57 PsychoKiller_Draft_58 PsychoKiller_Draft_59 PsychoKiller_Draft_60 PsychoKiller_Draft_61 PsychoKiller_Draft_62 PsychoKiller_Draft_63 PsychoKiller_Draft_64 PsychoKiller_Draft_65 PsychoKiller_Draft_66 PsychoKiller_Draft_67 PsychoKiller_Draft_68 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |