Gordonia phage Azira
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Detailed Information for Phage Azira
Discovery Information
Isolation HostGordonia rubripertincta NRRL B-16540
Found ByCoen McGarrah
Year Found2022
Location FoundTampa, FL United States
Finding InstitutionUniversity of South Florida
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates28.071111 N, 82.413056 W Map
Discovery NotesDate Collected: 9/5/22
Conditions: 91° F, cloudy, 61% humidity 
Location: Perimeter of Avalon Heights basketball courts 
Notes: Dark, damp, slightly sandy, taken from about ½ an inch under the surface
Naming NotesNamed after the character Aziraphale from Good Omens by Neil Gaiman and Terry Pratchet. Aziraphale guarded the eastern gate of Eden with a flaming sword.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 28, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage777
Genome length (bp)45336
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGTAGGCAT
GC Content61.9%
Fasta file available?Yes: Download fasta file
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUniversity of South Florida
Annotation StatusIn GenBank
Plaque NotesSmall, turbid plaques with rough edges. Plaques grow slightly larger in higher concentrations.
Number of Genes67
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOQ709211
Refseq NumberNone yet
Published in PaperMcGarrah et al 2023, Microbiology Resource Announcements: Isolation and Annotation of Azira, a CT Cluster Phage That Infects Gordonia rubripertincta
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.86x10^11 PFU/mL
Pitt Freezer Box# 149
Pitt Freezer Box Grid# D11
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload