| Detailed Information for Phage Blondies |
| Discovery Information |
| Isolation Host | Gordonia terrae CAG3 |
| Found By | Ariel Stean |
| Year Found | 2024 |
| Location Found | Manhattan, KS United States |
| Finding Institution | Kansas State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 23°C |
| GPS Coordinates | 39.1232 N, 96.344 W Map |
| Discovery Notes | The soil sample is from Meadowlark Hills. The dirt was very dry and was collected on a warmer day. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 15, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 8665 |
| Genome length (bp) | 46149 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTAGGCTT |
| GC Content | 60.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CT |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Lysogeny confirmed by doing the patch assay.
Infected other hosts: Gordonia lacunae and Gordonia rubripertincta |
| Other Cluster Members |
|
| Annotating Institution | Kansas State University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaque had a halo around it and was not smooth. |
| Morphotype | Siphoviridae |
| Number of Genes | 74 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PV940997 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.5x10^10 |
| Pitt Freezer Box# |
204 |
| Pitt Freezer Box Grid# |
D2 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
