| Detailed Information for Phage Blondies | |
| Discovery Information | |
| Isolation Host | Gordonia terrae CAG3 |
| Found By | Ariel Stean |
| Year Found | 2024 |
| Location Found | Manhattan, KS United States |
| Finding Institution | Kansas State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 23°C |
| GPS Coordinates | 39.1232 N, 96.344 W Map |
| Discovery Notes | This plaque was found at Meadowlark Hills. The dirt was very dry and was collected on a warmer day. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 15, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 8665 |
| Genome length (bp) | 46149 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTAGGCTT |
| GC Content | 60.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | CT |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Lysogeny confirmed by doing that patch assay. Infected other hosts: Gordonia Lawnai and Gordonia Rubripertincta DNA Extraction: 1.78, 260/280 DNA Concentration: 94.6 Titer: 1.5x10^10 |
| Other Cluster Members |
Click to ViewAgatha AikoCarson Amok AndPeggy Axym Azira Bavilard BigChungus BillDoor Biskit Blondies Burnsey Button Buttrmlkdreams CanesSauce Carsonalex CherryonLim ChickenTender ChocoMunchkin Cleo Cozz Dre3 Elinal Eliott Emalyn Feastonyeet Fribs8 Gibbous GiKK GoldHunter GTE2 Hexbug HippoPololi Horseradish Jamzy KayGee Lauer MAnor Margaret MaVan Mayweather MScarn MunkgeeRoachy Nibbles Nina Nodigi Orla Pons PotPie PsychoKiller Quasar RanchParmCat RedBaron SheckWes SketchMex Socotra Sopespian Starburst SteamedHams SummitAcademy Survivors SweatNTears Tolls Troje Typhonomachy Vine Yakult Yarn Yucky Yummy Zareef |
| Annotating Institution | Kansas State University |
| Annotation Status | Being Annotated (Expected completion by 5/1/2025) |
| Plaque Notes | Plaque had a halo around it and was not smooth. |
| Morphotype | Siphoviridae |
| Number of Genes | 73 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewBlondies_1 Blondies_2 Blondies_3 Blondies_4 Blondies_5 Blondies_6 Blondies_7 Blondies_8 Blondies_9 Blondies_10 Blondies_11 Blondies_12 Blondies_13 Blondies_14 Blondies_15 Blondies_16 Blondies_17 Blondies_18 Blondies_19 Blondies_20 Blondies_21 Blondies_22 Blondies_23 Blondies_24 Blondies_25 Blondies_26 Blondies_27 Blondies_28 Blondies_29 Blondies_30 Blondies_31 Blondies_32 Blondies_33 Blondies_34 Blondies_35 Blondies_36 Blondies_37 Blondies_38 Blondies_39 Blondies_40 Blondies_41 Blondies_42 Blondies_43 Blondies_44 Blondies_45 Blondies_46 Blondies_47 Blondies_48 Blondies_49 Blondies_50 Blondies_51 Blondies_52 Blondies_53 Blondies_54 Blondies_55 Blondies_56 Blondies_57 Blondies_58 Blondies_59 Blondies_60 Blondies_61 Blondies_62 Blondies_63 Blondies_64 Blondies_65 Blondies_66 Blondies_67 Blondies_68 Blondies_69 Blondies_70 Blondies_71 Blondies_72 Blondies_73 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 1.5x10^10 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |