| Detailed Information for Phage PsychoKiller | |
| Discovery Information | |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | Jon Mitchell |
| Year Found | 2020 |
| Location Found | Aberdeen, SD Brown |
| Finding Institution | Northern State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 25°C |
| GPS Coordinates | 45.712853 N, 98.275606 W Map |
| Discovery Notes | Environmental sample was taken from the wooded forest floor following a rain including several Ascomycete and Basidiomycete species attached to decaying/putrid branches/down trees. Luscious moss, sloppy soil, plant species, invertebrates, snails, bugs, rotten detritus. Very fertile soil sample in shade, small lighting between tree canopy. Birch, pine oak, maple, and ponderosa and white pine trees (needles and fallen/rotting leaves). Sample was harvested in September, 21 ~1:45 pm. Sample smells foul, actually beyond foul...very "juicy", "oozy" and "slimy". Quite dark. Failed to centrifuge distinctly and filtering with 0.22 micron slow and painful (use many filters and vacuum to recover ~10 microliters of sample. |
| Naming Notes | Naming this virus I was listening to The Talking Heads song of the same name. This bacteriophage had a pretty high titer and was most reliable at lysing host. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 19, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2098 |
| Genome length (bp) | 46085 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTAGGCTT |
| GC Content | 60.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | CT |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewAgatha AikoCarson Amok AndPeggy Axym Azira BigChungus BillDoor Biskit Burnsey Button Buttrmlkdreams Carsonalex CherryonLim Cleo Cozz Dre3 Elinal Eliott Emalyn Feastonyeet Fribs8 Gibbous GiKK GoldHunter GTE2 Hexbug HippoPololi Horseradish Jamzy KayGee Lauer MAnor Margaret MaVan Mayweather MScarn MunkgeeRoachy Nibbles Nina Nodigi Orla Pons PotPie PsychoKiller Quasar RanchParmCat RedBaron SheckWes SketchMex Sopespian SteamedHams SummitAcademy Survivors SweatNTears Tolls Troje Typhonomachy Vine Yakult Yarn Yummy Zareef |
| Annotating Institution | Northern State University |
| Annotation Status | Being Annotated (Expected completion by 12/31/2024) |
| Plaque Notes | Lytic and round on Gordonia. 1-2 mm in diameter. Sometimes tiny. Sometime opaque also. |
| Number of Genes | 68 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewPsychoKiller_1 PsychoKiller_2 PsychoKiller_3 PsychoKiller_4 PsychoKiller_5 PsychoKiller_6 PsychoKiller_7 PsychoKiller_8 PsychoKiller_9 PsychoKiller_10 PsychoKiller_11 PsychoKiller_12 PsychoKiller_13 PsychoKiller_14 PsychoKiller_15 PsychoKiller_16 PsychoKiller_17 PsychoKiller_18 PsychoKiller_19 PsychoKiller_20 PsychoKiller_21 PsychoKiller_22 PsychoKiller_23 PsychoKiller_24 PsychoKiller_25 PsychoKiller_26 PsychoKiller_27 PsychoKiller_28 PsychoKiller_29 PsychoKiller_30 PsychoKiller_31 PsychoKiller_32 PsychoKiller_33 PsychoKiller_34 PsychoKiller_35 PsychoKiller_36 PsychoKiller_37 PsychoKiller_38 PsychoKiller_39 PsychoKiller_40 PsychoKiller_41 PsychoKiller_42 PsychoKiller_43 PsychoKiller_44 PsychoKiller_45 PsychoKiller_46 PsychoKiller_47 PsychoKiller_48 PsychoKiller_49 PsychoKiller_50 PsychoKiller_51 PsychoKiller_52 PsychoKiller_53 PsychoKiller_54 PsychoKiller_55 PsychoKiller_56 PsychoKiller_57 PsychoKiller_58 PsychoKiller_59 PsychoKiller_60 PsychoKiller_61 PsychoKiller_62 PsychoKiller_63 PsychoKiller_64 PsychoKiller_65 PsychoKiller_66 PsychoKiller_67 PsychoKiller_68 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |