| Detailed Information for Phage SweatNTears |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Jacob Cote, Ben Gove |
| Year Found | 2018 |
| Location Found | ME USA |
| Finding Institution | University of Maine, Honors College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 44.588706 N, 69.293424 W Map |
| Discovery Notes | Isolated from soil that containing donkey manure compost. Sample taken from the Common Ground Fair in Unity, Maine. |
| Naming Notes | The first phage we isolated after many, many direct and enriched isolation attempts with other soil samples. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 15, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 637 |
| Genome length (bp) | 45917 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTAGGCTT |
| GC Content | 59.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CT |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Efficiency of lysogeny calculated to be 42.1% in G.terrae. Host range includes G. terrae, Rhodococcus erythropolis, and potentially Gordonia rubripertincta. |
| Other Cluster Members |
|
| Annotating Institution | University of Maine, Honors College |
| Annotation Status | In GenBank |
| Plaque Notes | Small, pinpoint plaques with turbid halos around a fully cleared center. Variability in plaque morphology has been observed at high lysate concentrations. |
| Morphotype | Siphoviridae |
| Number of Genes | 71 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK967383 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
85 |
| Pitt Freezer Box Grid# |
A5 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
