| Detailed Information for Phage Button | |
| Discovery Information | |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | Zoe Aurelia Meyer |
| Year Found | 2021 |
| Location Found | Cullowhee, NC United States of America |
| Finding Institution | Western Carolina University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 16°C |
| GPS Coordinates | 35.3103 N, 83.1875 W Map |
| Discovery Notes | This Phage was found in the Cullowhee Creek on a rainy day under the bridge near the baseball fields. It was in a shallow part of the river. This sample is a water sample so the procedures to originally harvest it from the sample are a bit different than that of a soil sample. |
| Naming Notes | I named this phage after the nickname my Dad calls me. He is the one who inspired me to pursue and be passionate about the sciences. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 8, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 826 |
| Genome length (bp) | 46090 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTAGGCAT |
| GC Content | 62.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | CT |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewAgatha AikoCarson Amok AndPeggy Axym Azira BigChungus BillDoor Biskit Burnsey Button Buttrmlkdreams Carsonalex CherryonLim Cleo Cozz Dre3 Elinal Eliott Emalyn Feastonyeet Fribs8 Gibbous GiKK GoldHunter GTE2 Hexbug HippoPololi Horseradish Jamzy KayGee Lauer MAnor Margaret MaVan Mayweather MScarn MunkgeeRoachy Nibbles Nina Nodigi Orla Pons PotPie PsychoKiller Quasar RanchParmCat RedBaron SheckWes SketchMex Sopespian SteamedHams SummitAcademy Survivors SweatNTears Tolls Troje Typhonomachy Vine Yakult Yarn Yummy Zareef |
| Annotating Institution | Western Carolina University |
| Annotation Status | In GenBank |
| Plaque Notes | This phage will plaque relatively small compared to other phages using a different bacterial host. Button also has a halo surrounding it most of the time, which can be noticeably seen on spot tests and spot titers. The phage also presents as having turbidity towards the edges of the plaque and clears up towards the most concentrated middle part. |
| Number of Genes | 71 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewButton_1 Button_2 Button_3 Button_4 Button_5 Button_6 Button_7 Button_8 Button_9 Button_10 Button_11 Button_12 Button_13 Button_14 Button_15 Button_16 Button_17 Button_18 Button_19 Button_20 Button_21 Button_22 Button_23 Button_24 Button_25 Button_26 Button_27 Button_28 Button_29 Button_30 Button_31 Button_32 Button_33 Button_34 Button_35 Button_36 Button_37 Button_38 Button_39 Button_40 Button_41 Button_42 Button_43 Button_44 Button_45 Button_46 Button_47 Button_48 Button_49 Button_50 Button_51 Button_52 Button_53 Button_54 Button_55 Button_56 Button_57 Button_58 Button_59 Button_60 Button_61 Button_62 Button_63 Button_64 Button_65 Button_66 Button_67 Button_68 Button_69 Button_70 Button_71 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON970621 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 5x10^9 PFU/mL |
| Pitt Freezer Box# | 138 |
| Pitt Freezer Box Grid# | H7 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
