| Detailed Information for Phage Emotion | |
| Discovery Information | |
| Isolation Host | Arthrobacter sulfureus NRRL B-14730 |
| Found By | Sara Barcik Weissman |
| Year Found | 2022 |
| Location Found | Lakewood, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 33.830741 N, 118.163527 W Map |
| Discovery Notes | Isolated by TA Dominic Garza. Isolated using mixed culture method. An enriched culture was prepared by combining 15 mL of soil with 25 mL of PYCA broth. The bacterial strains of A. sp., A. globiformis, A. sulfureus and A. atrocyaneus were then added in a ratio of 1:5:192:588 respectively. The enriched culture was incubated at 30℃ for 48 hours with shaking. After filtering with a 0.22 µm filter, the sample was plated on PYCA plates with 2X PYCa soft agar using the standard pour plate method. Sample description: The soil sample was slightly moist and taken from an urban/industrial area. The collection site was next to a parking lot, but there was little to no foot traffic. There were several pieces of rotting fruit (an orange and banana peel) next to the soil collection site. |
| Naming Notes | The name is a direct reference to the album Emotion – produced by singer/songwriter Carly Rae Jepsen. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Sep 2, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 732 |
| Genome length (bp) | 45092 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAAGCGGCAT |
| GC Content | 67.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AZ |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to ViewAdolin Adumb2043 AEgle AGrandiflora Amyev Asa16 Ascela Berrie Cassia Community Crewmate Cyan DrManhattan DrSierra Elezi Emotion Eraser Iter Janeemi Joemato JohnDoe Jstan Kaylissa KeAlii Lego Liebe Lizalica London MaGuCo Maureen Mudpuppy Niobe Nitro ObiToo Phives Pixelle Powerpuff Reedo Simpson Snek Tallboi Tbone TforTroy Tian Tuck Tutumahutu Tweety19 VResidence VroomVroom Warda Wildwest Yang YesChef |
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | In GenBank |
| Plaque Notes | Description of plaque morphology: Clear center with turbid edges Average diameter: 1 mm |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewEmotion_1 Emotion_2 Emotion_3 Emotion_4 Emotion_5 Emotion_6 Emotion_7 Emotion_8 Emotion_9 Emotion_10 Emotion_11 Emotion_12 Emotion_13 Emotion_14 Emotion_15 Emotion_16 Emotion_17 Emotion_18 Emotion_19 Emotion_20 Emotion_21 Emotion_22 Emotion_23 Emotion_24 Emotion_25 Emotion_26 Emotion_27 Emotion_28 Emotion_29 Emotion_30 Emotion_31 Emotion_32 Emotion_33 Emotion_34 Emotion_35 Emotion_36 Emotion_37 Emotion_38 Emotion_39 Emotion_40 Emotion_41 Emotion_42 Emotion_43 Emotion_44 Emotion_45 Emotion_46 Emotion_47 Emotion_48 Emotion_49 Emotion_50 Emotion_51 Emotion_52 Emotion_53 Emotion_54 Emotion_55 Emotion_56 Emotion_57 Emotion_58 Emotion_59 Emotion_60 Emotion_61 Emotion_62 Emotion_63 Emotion_64 Emotion_65 Emotion_66 Emotion_67 Emotion_68 Emotion_69 Emotion_70 Emotion_72 Emotion_73 Emotion_74 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OQ709216 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 155 |
| Pitt Freezer Box Grid# | G11 |
| Available Files | |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |