Arthrobacter phage Emotion
Emotion EM Picture
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Detailed Information for Phage Emotion
Discovery Information
Isolation HostArthrobacter sulfureus ATCC 19098
Found BySara Barcik Weissman
Year Found2022
Location FoundLakewood, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates33.830741 N, 118.163527 W Map
Discovery NotesIsolated by TAs Dominic Garza and Michelle Zorawik.
Isolated using mixed culture method.

An enriched culture was prepared by combining 15 mL of soil with 25 mL of PYCA broth. The bacterial strains of A. sp., A. globiformis, A. sulfureus and A. atrocyaneus were then added in a ratio of 1:5:192:588 respectively. The enriched culture was incubated at 30℃ for 48 hours with shaking. After filtering with a 0.22 µm filter, the sample was plated on PYCA plates with 2X PYCa soft agar using the standard pour plate method.

Sample description: The soil sample was slightly moist and taken from an urban/industrial area. The collection site was next to a parking lot, but there was little to no foot traffic. There were several pieces of rotting fruit (an orange and banana peel) next to the soil collection site.
Naming NotesThe name is a direct reference to the album Emotion – produced by singer/songwriter Carly Rae Jepsen.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedSep 2, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage732
Genome length (bp)45092
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGAAGCGGCAT
GC Content67.9%
Fasta file available?Yes: Download fasta file
Cluster Life CycleTemperate
Lysogeny NotesUnlike the majority of Cluster AZ members, Emotion does not have an identifiable integrase gene.
Other Cluster Members
Annotating InstitutionUniversity of California, Los Angeles
Annotation StatusIn GenBank
Plaque NotesDescription of plaque morphology: Clear center with turbid edges
Average diameter: 1 mm
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOQ709216
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 155
Pitt Freezer Box Grid# G10
Available Files
Plaque PictureDownload
GenBank File for PhameratorDownload