Arthrobacter phage Emotion
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Detailed Information for Phage Emotion
Discovery Information
Isolation HostArthrobacter sulfureus NRRL B-14730
Found ByKevin Olvera
Year Found2022
Location FoundLos Angeles, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates34.069167 N, 118.451667 W Map
Discovery NotesIsolated by TA Dominic Garza.
Isolated using mixed culture method.

An enriched culture was prepared by combining 15 mL of soil with 25 mL of PYCA broth. The bacterial strains of A. sp., A. globiformis, A. sulfureus and A. atrocyaneus were then added in a ratio of 1:5:192:588 respectively. The enriched culture was incubated at 30℃ for 48 hours with shaking. After filtering with a 0.22 µm filter, the sample was plated on PYCA plates with 2X PYCa soft agar using the standard pour plate method.

Sample description: The soil sample was half dry and half moist. The collection site was urban. It was collected at the base of a tree right outside of an apartment complex. There was adequate access to water with drippings falling from the tree above. The collection site was not sloped.
Naming NotesThe name is a direct reference to the album Emotion – produced by singer/songwriter Carly Rae Jepsen.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedSep 2, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage732
Genome length (bp)45092
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGAAGCGGCAT
GC Content67.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUniversity of California, Los Angeles
Annotation StatusBeing Annotated (Expected completion by 3/2/2023)
Plaque NotesDescription of plaque morphology: Turbid with fuzzy edges
Average diameter: 1.5 mm
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload