| Detailed Information for Phage Emotion |
| Discovery Information |
| Isolation Host | Arthrobacter sulfureus ATCC 19098 |
| Found By | Sara Barcik Weissman |
| Year Found | 2022 |
| Location Found | Lakewood, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 33.830741 N, 118.163527 W Map |
| Discovery Notes | Isolated by TAs Dominic Garza and Michelle Zorawik.
Isolated using mixed culture method.
An enriched culture was prepared by combining 15 mL of soil with 25 mL of PYCA broth. The bacterial strains of A. sp., A. globiformis, A. sulfureus and A. atrocyaneus were then added in a ratio of 1:5:192:588 respectively. The enriched culture was incubated at 30℃ for 48 hours with shaking. After filtering with a 0.22 µm filter, the sample was plated on PYCA plates with 2X PYCa soft agar using the standard pour plate method.
Sample description: The soil sample was slightly moist and taken from an urban/industrial area. The collection site was next to a parking lot, but there was little to no foot traffic. There were several pieces of rotting fruit (an orange and banana peel) next to the soil collection site. |
| Naming Notes | The name is a direct reference to the album Emotion – produced by singer/songwriter Carly Rae Jepsen. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Sep 2, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 732 |
| Genome length (bp) | 45092 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAAGCGGCAT |
| GC Content | 67.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AZ |
| Subcluster | AZ4 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Unlike the majority of Cluster AZ members, Emotion does not have an identifiable integrase gene. |
| Other Cluster Members |
|
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | In GenBank |
| Plaque Notes | Description of plaque morphology: Clear center with turbid edges
Average diameter: 1 mm |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OQ709216 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
155 |
| Pitt Freezer Box Grid# |
G11 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
