The Actinobacteriophage Database

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Detailed Information for Phage Lego
Discovery Information
Isolation Host	Arthrobacter globiformis NRRL B-2880
Former names	Legolise
Found By	Eddie Briseno
Year Found	2019
Location Found	Santa Monica, CA USA
Finding Institution	University of California, Los Angeles
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	30°C
GPS Coordinates	34.016253 N, 118.501056 W Map
Discovery Notes	Arthrobacter phage Lego was isolated from soil by enrichment plating at 30°C. The sample was collected by Eddie Briseno in Santa Monica, CA. Legolise was isolated, characterized, and annotated by An Ta, Ashley Min, Eddie Briseno, Maya Mendez, Esther Choe, Byron Corado, Emily Farag, Rachel Smith. Instructor: Amanda Freise Teaching Assistant: Canela Torres
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 2, 2020
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Sequencer Used	Illumina MiSeq
Read Type	Single-end reads
Read Length	150 bp
Approximate Shotgun Coverage	3383
Genome length (bp)	43446
Character of genome ends	3' Sticky Overhang
Overhang Length	11 bases
Overhang Sequence	CGAAGGGGCAT
GC Content	67.5%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	AZ
Subcluster	AZ1
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Adolin (AZ1) Adumb2043 (AZ1) AEgle (AZ1) AGrandiflora (AZ1) Amyev (AZ1) Asa16 (AZ1) Ascela (AZ1) Berrie (AZ1) Cassia (AZ1) Community (AZ1) Crewmate (AZ1) Cyan (AZ1) DrManhattan (AZ1) DrSierra (AZ1) Elezi (AZ1) Eraser (AZ1) Iter (AZ1) IttyBittyPiggy (AZ1) Janeemi (AZ1) Joemato (AZ1) JohnDoe (AZ1) Jstan (AZ1) JuneStar (AZ1) Kaylissa (AZ1) KeAlii (AZ1) Lego (AZ1) Lizalica (AZ1) London (AZ1) MissSwiss (AZ1) Mudpuppy (AZ1) Niobe (AZ1) Nitro (AZ1) ObiToo (AZ1) Phives (AZ1) Pixelle (AZ1) Powerpuff (AZ1) Pumpkins (AZ1) Reedo (AZ1) Schaffner (AZ1) Simpson (AZ1) Skelbel (AZ1) Subaru (AZ1) Sue2 (AZ1) Tallboi (AZ1) Tbone (AZ1) TforTroy (AZ1) Tian (AZ1) Tuck (AZ1) Turab (AZ1) Tutumahutu (AZ1) VResidence (AZ1) Warda (AZ1) Wildwest (AZ1) Yang (AZ1) YesChef (AZ1) Liebe (AZ2) MaGuCo (AZ2) Maureen (AZ2) Snek (AZ3) Tweety19 (AZ3) Emotion (AZ4) JasmineDragon (AZ4) LadyAstra (AZ4) MiniMommy (AZ4) ShakeItOph (AZ4) VroomVroom (AZ4) Ashes (AZ5) Beaupre (AZ5) Cappuccino (AZ5) Donkey (AZ5) Gambol (AZ5) Giorgio (AZ5) Gumpizza (AZ5) Halsey (AZ5) Kalimba (AZ5) Moss (AZ5) Mysterium (AZ5) RockScotty (AZ5) Sabourin (AZ5) Sooty (AZ5) SpecialK (AZ5) Stuu (AZ5) Exile (AZ6) Soondubu (AZ6) AinMach (AZ7)
Annotating Institution	University of California, Los Angeles
Annotation Status	In GenBank
Morphotype	Siphoviridae
Number of Genes	69
Number of tRNAs	0
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Lego_1 (terminase small subunit) Lego_2 (terminase large subunit) Lego_3 (portal protein) Lego_4 (capsid maturation protease and VIP2-like ADP-ribosyltransferase toxin) Lego_5 Lego_6 (scaffolding protein) Lego_7 (major capsid protein) Lego_8 (head-to-tail adaptor) Lego_9 Lego_10 (head-to-tail stopper) Lego_11 Lego_12 (tail terminator) Lego_13 (major tail protein) Lego_14 (tail assembly chaperone) Lego_15 (tail assembly chaperone) Lego_16 (tape measure protein) Lego_17 (minor tail protein) Lego_18 (minor tail protein) Lego_19 (minor tail protein) Lego_20 (minor tail protein) Lego_21 Lego_22 Lego_23 Lego_24 (deoxynucleoside monophosphate kinase) Lego_25 Lego_26 (exonuclease) Lego_27 (nucleoside deoxyribosyltransferase) Lego_28 (LAGLIDADG endonuclease) Lego_29 (recombination directionality factor) Lego_30 Lego_31 Lego_32 Lego_33 (NrdH-like glutaredoxin) Lego_34 Lego_35 (holliday junction resolvase) Lego_36 Lego_37 (DNA methyltransferase) Lego_38 (DNA primase/helicase) Lego_39 Lego_40 Lego_41 (DNA polymerase I) Lego_42 (DNA ligase) Lego_43 Lego_44 (DNA binding protein) Lego_45 (DNA binding protein) Lego_46 (SprT-like protease) Lego_47 Lego_48 Lego_49 (serine integrase) Lego_50 Lego_51 (RNA binding protein) Lego_52 Lego_53 Lego_54 Lego_55 Lego_56 Lego_57 (endolysin) Lego_58 Lego_59 Lego_60 Lego_61 Lego_62 Lego_63 Lego_64 Lego_65 Lego_66 Lego_67 Lego_68 Lego_69 (HNH endonuclease)
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MT024869
Refseq Number	None yet
Published in Paper	Kapinos et al 2022, PLOS ONE: Novel Cluster AZ Arthrobacter phages Powerpuff, Lego, and YesChef exhibit close functional relationships with Microbacterium phages
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	116
Pitt Freezer Box Grid#	C8
Available Files
EM Picture	Download
GenBank File for Phamerator	Download

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