Arthrobacter phage Lego
Lego Plaque Picture
Lego EM Picture
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(A. sp. ATCC 21022)
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Detailed Information for Phage Lego
Discovery Information
Isolation HostArthrobacter globiformis B-2979
Former namesLegolise
Found ByEddie Briseno
Year Found2019
Location FoundSanta Monica, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates34.016253 N, 118.501056 W Map
Discovery NotesArthrobacter phage Lego was isolated from soil by enrichment plating at 30°C. The sample was collected by Eddie Briseno in Santa Monica, CA. Legolise was isolated, characterized, and annotated by An Ta, Ashley Min, Eddie Briseno, Maya Mendez, Esther Choe, Byron Corado, Emily Farag, Rachel Smith.

Instructor: Amanda Freise
Teaching Assistant: Canela Torres
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 2, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage3383
Genome length (bp)43446
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGAAGGGGCAT
GC Content67.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterAZ
SubclusterAZ1
Cluster Life CycleTemperate
Other Cluster Members

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Adolin (AZ1)

Adumb2043 (AZ1)

AEgle (AZ1)

AGrandiflora (AZ1)

Amyev (AZ1)

Asa16 (AZ1)

Ascela (AZ1)

Berrie (AZ1)

Cassia (AZ1)

Community (AZ1)

Crewmate (AZ1)

Cyan (AZ1)

DrManhattan (AZ1)

DrSierra (AZ1)

Elezi (AZ1)

Eraser (AZ1)

Iter (AZ1)

Janeemi (AZ1)

Joemato (AZ1)

JohnDoe (AZ1)

Jstan (AZ1)

Kaylissa (AZ1)

KeAlii (AZ1)

Lego (AZ1)

Lizalica (AZ1)

London (AZ1)

MissSwiss (AZ1)

Mudpuppy (AZ1)

Niobe (AZ1)

Nitro (AZ1)

ObiToo (AZ1)

Phives (AZ1)

Pixelle (AZ1)

Powerpuff (AZ1)

Reedo (AZ1)

Simpson (AZ1)

Tallboi (AZ1)

Tbone (AZ1)

TforTroy (AZ1)

Tian (AZ1)

Tuck (AZ1)

Tutumahutu (AZ1)

VResidence (AZ1)

Warda (AZ1)

Wildwest (AZ1)

Yang (AZ1)

YesChef (AZ1)

Liebe (AZ2)

MaGuCo (AZ2)

Maureen (AZ2)

Snek (AZ3)

Tweety19 (AZ3)

Emotion (AZ4)

JasmineDragon (AZ4)

MiniMommy (AZ4)

VroomVroom (AZ4)
Annotating InstitutionUniversity of California, Los Angeles
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Number of Genes69
Has been Phamerated?Yes
Gene List

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Lego_1
(terminase small subunit)

Lego_2
(terminase large subunit)

Lego_3
(portal protein)

Lego_4
(capsid maturation protease and VIP2-like ADP-ribosyltransferase toxin)

Lego_5

Lego_6
(scaffolding protein)

Lego_7
(major capsid protein)

Lego_8
(head-to-tail adaptor)

Lego_9

Lego_10
(head-to-tail stopper)

Lego_11

Lego_12
(tail terminator)

Lego_13
(major tail protein)

Lego_14
(tail assembly chaperone)

Lego_15
(tail assembly chaperone)

Lego_16
(tape measure protein)

Lego_17
(minor tail protein)

Lego_18
(minor tail protein)

Lego_19
(minor tail protein)

Lego_20
(minor tail protein)

Lego_21

Lego_22

Lego_23

Lego_24
(deoxynucleoside monophosphate kinase)

Lego_25

Lego_26
(exonuclease)

Lego_27
(nucleoside deoxyribosyltransferase)

Lego_28
(LAGLIDADG endonuclease)

Lego_29
(recombination directionality factor)

Lego_30

Lego_31

Lego_32

Lego_33
(NrdH-like glutaredoxin)

Lego_34

Lego_35
(holliday junction resolvase)

Lego_36

Lego_37
(DNA methyltransferase)

Lego_38
(DNA primase/helicase)

Lego_39

Lego_40

Lego_41
(DNA polymerase I)

Lego_42
(DNA ligase)

Lego_43

Lego_44
(DNA binding protein)

Lego_45
(DNA binding protein)

Lego_46
(SprT-like protease)

Lego_47

Lego_48

Lego_49
(serine integrase)

Lego_50

Lego_51
(RNA binding protein)

Lego_52

Lego_53

Lego_54

Lego_55

Lego_56

Lego_57
(endolysin)

Lego_58

Lego_59

Lego_60

Lego_61

Lego_62

Lego_63

Lego_64

Lego_65

Lego_66

Lego_67

Lego_68

Lego_69
(HNH endonuclease)
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT024869
Refseq NumberNone yet
Published in PaperKapinos et al 2022, PLOS ONE: Novel Cluster AZ Arthrobacter phages Powerpuff, Lego, and YesChef exhibit close functional relationships with Microbacterium phages
Archiving Info
Archiving status Archived
Pitt Freezer Box# 116
Pitt Freezer Box Grid# C8
Available Files
EM PictureDownload
GenBank File for PhameratorDownload