Arthrobacter phage Asa16
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Detailed Information for Phage Asa16
Discovery Information
Isolation HostArthrobacter globiformis B-2979
Found ByAlia Abdulla
Year Found2020
Location FoundNew Haven, CT United States
Finding InstitutionSouthern Connecticut State University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates41.3347 N, 72.9421 W Map
Discovery NotesThere was a rainstorm a few days before the soil collection happened. On the day of collection, it was sunny, however, sunlight did not shine on the collection area. When the soil was taken, the outside temperature was 17.8 degrees Celsius. Also, the site was next to a trail, which looked as if it was hardly used. At the collection site, 20 millimeters were dug into the ground with a spatula, and 15 milliliters of the soil was taken. The soil was damp and was surrounded by decomposing leaves and sticks. The collection happened around 9:04 AM on August 31, 2020. This was the second soil sample collected that day.
Naming NotesI wanted the name of my bacteriophage to be significant, so I decided to name it "Asa16." "Asa" has a meaning in multiple languages. A few of the languages are Hebrew, where it means healer; Japanese, where it means morning (symbolic for new beginnings); and Persian, where it means powerful and beautiful. "16" in the name represents the age I was when I collected the bacteriophage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 7, 2021
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2348
Genome length (bp)43601
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGAAGGGGCAT
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterAZ
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionSouthern Connecticut State University
Annotation StatusIn GenBank
Plaque NotesMedium plaques with an average diameter of 4.74 mm. The center of each plaque is clear with a turbid ring surrounding it.
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMZ681506
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 3.9e9
Pitt Freezer Box# 129
Pitt Freezer Box Grid# F2
Available Files
Plaque PictureDownload
GenBank File for PhameratorDownload