| Discovery Information |
| Isolation Host | Arthrobacter globiformis NRRL B-2880 |
| Found By | Alia Abdulla |
| Year Found | 2020 |
| Location Found | New Haven, CT United States |
| Finding Institution | Southern Connecticut State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 41.3347 N, 72.9421 W Map |
| Discovery Notes | There was a rainstorm a few days before the soil collection happened. On the day of collection, it was sunny, however, sunlight did not shine on the collection area. When the soil was taken, the outside temperature was 17.8 degrees Celsius. Also, the site was next to a trail, which looked as if it was hardly used. At the collection site, 20 millimeters were dug into the ground with a spatula, and 15 milliliters of the soil was taken. The soil was damp and was surrounded by decomposing leaves and sticks. The collection happened around 9:04 AM on August 31, 2020. This was the second soil sample collected that day. |
| Naming Notes | I wanted the name of my bacteriophage to be significant, so I decided to name it "Asa16." "Asa" has a meaning in multiple languages. A few of the languages are Hebrew, where it means healer; Japanese, where it means morning (symbolic for new beginnings); and Persian, where it means powerful and beautiful. "16" in the name represents the age I was when I collected the bacteriophage. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 7, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2348 |
| Genome length (bp) | 43601 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAAGGGGCAT |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AZ |
| Subcluster | AZ1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Southern Connecticut State University |
| Annotation Status | In GenBank |
| Plaque Notes | Medium plaques with an average diameter of 4.74 mm. The center of each plaque is clear with a turbid ring surrounding it. |
| Number of Genes | 70 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MZ681506 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
3.9e9 |
| Pitt Freezer Box# |
129 |
| Pitt Freezer Box Grid# |
F2 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
