| Detailed Information for Phage Tian |
| Discovery Information |
| Isolation Host | Arthrobacter globiformis NRRL B-2880 |
| Found By | Shay Patel |
| Year Found | 2022 |
| Location Found | Ottawa, Canada |
| Finding Institution | University of Ottawa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 12°C |
| GPS Coordinates | 45.36963 N, 75.62454 W Map |
| Discovery Notes | 5 mL of loamy soil was extracted from a garden bed at 18 Upwood Street in Ottawa, Ontario, Canada. The soil was extracted at a depth of 5 cm below the surface. |
| Naming Notes | The actinobacteriophage in question was named after my younger cousin. He played a very important role in my childhood and has been a role model throughout the entirety of my life. In order to demonstrate my appreciation, I named my phage after him. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 6, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 226 |
| Genome length (bp) | 44283 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAAGGGGCAT |
| GC Content | 67.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AZ |
| Subcluster | AZ1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Ottawa |
| Annotation Status | Being Annotated (Expected completion by 5/1/2024) |
| Plaque Notes | The plaques produced by actinobacteriophage Tian are 0.75 cm in diameter and have both turbid and transparent regions. They have a bullseye appearance; the center of the plaque is transparent while the periphery of the plaque is turbid. |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
154 |
| Pitt Freezer Box Grid# |
H8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
