| Detailed Information for Phage Tian | |
| Discovery Information | |
| Isolation Host | Arthrobacter globiformis NRRL B-2880 |
| Found By | Shay Patel |
| Year Found | 2022 |
| Location Found | Ottawa, Canada |
| Finding Institution | University of Ottawa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 12°C |
| GPS Coordinates | 45.36963 N, 75.62454 W Map |
| Discovery Notes | 5 mL of loamy soil was extracted from a garden bed at 18 Upwood Street in Ottawa, Ontario, Canada. The soil was extracted at a depth of 5 cm below the surface. |
| Naming Notes | The actinobacteriophage in question was named after my younger cousin. He played a very important role in my childhood and has been a role model throughout the entirety of my life. In order to demonstrate my appreciation, I named my phage after him. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 6, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 226 |
| Genome length (bp) | 44283 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAAGGGGCAT |
| GC Content | 67.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AZ |
| Subcluster | AZ1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to ViewAinMach (None) Exile (None) Soondubu (None) Adolin (AZ1) Adumb2043 (AZ1) AEgle (AZ1) AGrandiflora (AZ1) Amyev (AZ1) Asa16 (AZ1) Ascela (AZ1) Berrie (AZ1) Cassia (AZ1) Community (AZ1) Crewmate (AZ1) Cyan (AZ1) DrManhattan (AZ1) DrSierra (AZ1) Elezi (AZ1) Eraser (AZ1) Iter (AZ1) IttyBittyPiggy (AZ1) Janeemi (AZ1) Joemato (AZ1) JohnDoe (AZ1) Jstan (AZ1) JuneStar (AZ1) Kaylissa (AZ1) KeAlii (AZ1) Lego (AZ1) Lizalica (AZ1) London (AZ1) MissSwiss (AZ1) Mudpuppy (AZ1) Niobe (AZ1) Nitro (AZ1) ObiToo (AZ1) Phives (AZ1) Pixelle (AZ1) Powerpuff (AZ1) Pumpkins (AZ1) Reedo (AZ1) Schaffner (AZ1) Simpson (AZ1) Skelbel (AZ1) Subaru (AZ1) Sue2 (AZ1) Tallboi (AZ1) Tbone (AZ1) TforTroy (AZ1) Tian (AZ1) Tuck (AZ1) Turab (AZ1) Tutumahutu (AZ1) VResidence (AZ1) Warda (AZ1) Wildwest (AZ1) Yang (AZ1) YesChef (AZ1) Liebe (AZ2) MaGuCo (AZ2) Maureen (AZ2) Snek (AZ3) Tweety19 (AZ3) Emotion (AZ4) JasmineDragon (AZ4) LadyAstra (AZ4) MiniMommy (AZ4) ShakeItOph (AZ4) VroomVroom (AZ4) Ashes (AZ5) Beaupre (AZ5) Cappuccino (AZ5) Donkey (AZ5) Gambol (AZ5) Giorgio (AZ5) Gumpizza (AZ5) Halsey (AZ5) Kalimba (AZ5) Moss (AZ5) Mysterium (AZ5) RockScotty (AZ5) Sabourin (AZ5) Sooty (AZ5) SpecialK (AZ5) Stuu (AZ5) |
| Annotating Institution | University of Ottawa |
| Annotation Status | Overdue (Expected completion by 8/31/2024) |
| Plaque Notes | The plaques produced by actinobacteriophage Tian are 0.75 cm in diameter and have both turbid and transparent regions. They have a bullseye appearance; the center of the plaque is transparent while the periphery of the plaque is turbid. |
| Number of Genes | 68 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewTian_1 Tian_2 Tian_3 Tian_4 Tian_5 Tian_6 Tian_7 Tian_8 Tian_9 Tian_10 Tian_11 Tian_12 Tian_13 Tian_14 Tian_15 Tian_16 Tian_17 Tian_18 Tian_19 Tian_20 Tian_21 Tian_22 Tian_23 Tian_24 Tian_25 Tian_26 Tian_27 Tian_28 Tian_29 Tian_30 Tian_31 Tian_32 Tian_33 Tian_34 Tian_35 Tian_36 Tian_37 Tian_38 Tian_39 Tian_40 Tian_41 Tian_42 Tian_43 Tian_44 Tian_45 Tian_46 Tian_47 Tian_48 Tian_49 Tian_50 Tian_51 Tian_52 Tian_53 Tian_54 Tian_55 Tian_56 Tian_57 Tian_58 Tian_59 Tian_60 Tian_61 Tian_62 Tian_63 Tian_64 Tian_65 Tian_66 Tian_67 Tian_68 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 154 |
| Pitt Freezer Box Grid# | H8 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
