| Detailed Information for Phage Tian | |
| Discovery Information | |
| Isolation Host | Arthrobacter globiformis NRRL B-2880 |
| Found By | Shay Patel |
| Year Found | 2022 |
| Location Found | Ottawa, Canada |
| Finding Institution | University of Ottawa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 12°C |
| GPS Coordinates | 45.36963 N, 75.62454 W Map |
| Discovery Notes | 5 mL of loamy soil was extracted from a garden bed at 18 Upwood Street in Ottawa, Ontario, Canada. The soil was extracted at a depth of 5 cm below the surface. |
| Naming Notes | The actinobacteriophage in question was named after my younger cousin. He played a very important role in my childhood and has been a role model throughout the entirety of my life. In order to demonstrate my appreciation, I named my phage after him. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 6, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 226 |
| Genome length (bp) | 44283 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAAGGGGCAT |
| GC Content | 67.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AZ |
| Subcluster | AZ1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to ViewAdolin (AZ1) Adumb2043 (AZ1) AEgle (AZ1) AGrandiflora (AZ1) Amyev (AZ1) Asa16 (AZ1) Ascela (AZ1) Berrie (AZ1) Cassia (AZ1) Community (AZ1) Crewmate (AZ1) Cyan (AZ1) DrManhattan (AZ1) DrSierra (AZ1) Elezi (AZ1) Eraser (AZ1) Iter (AZ1) IttyBittyPiggy (AZ1) Janeemi (AZ1) Joemato (AZ1) JohnDoe (AZ1) Jstan (AZ1) Kaylissa (AZ1) KeAlii (AZ1) Lego (AZ1) Lizalica (AZ1) London (AZ1) MissSwiss (AZ1) Mudpuppy (AZ1) Niobe (AZ1) Nitro (AZ1) ObiToo (AZ1) Phives (AZ1) Pixelle (AZ1) Powerpuff (AZ1) Reedo (AZ1) Simpson (AZ1) Tallboi (AZ1) Tbone (AZ1) TforTroy (AZ1) Tian (AZ1) Tuck (AZ1) Tutumahutu (AZ1) VResidence (AZ1) Warda (AZ1) Wildwest (AZ1) Yang (AZ1) YesChef (AZ1) Liebe (AZ2) MaGuCo (AZ2) Maureen (AZ2) Snek (AZ3) Tweety19 (AZ3) Emotion (AZ4) JasmineDragon (AZ4) MiniMommy (AZ4) VroomVroom (AZ4) |
| Annotating Institution | University of Ottawa |
| Annotation Status | Overdue (Expected completion by 5/1/2023) |
| Plaque Notes | The plaques produced by actinobacteriophage Tian are 0.75 cm in diameter and have both turbid and transparent regions. They have a bullseye appearance; the center of the plaque is transparent while the periphery of the plaque is turbid. |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewTian_Draft_1 Tian_Draft_2 Tian_Draft_3 Tian_Draft_4 Tian_Draft_5 Tian_Draft_6 Tian_Draft_7 Tian_Draft_8 Tian_Draft_9 Tian_Draft_10 Tian_Draft_11 Tian_Draft_12 Tian_Draft_13 Tian_Draft_14 Tian_Draft_15 Tian_Draft_16 Tian_Draft_17 Tian_Draft_18 Tian_Draft_19 Tian_Draft_20 Tian_Draft_21 Tian_Draft_22 Tian_Draft_23 Tian_Draft_24 Tian_Draft_25 Tian_Draft_26 Tian_Draft_27 Tian_Draft_28 Tian_Draft_29 Tian_Draft_30 Tian_Draft_31 Tian_Draft_32 Tian_Draft_33 Tian_Draft_34 Tian_Draft_35 Tian_Draft_36 Tian_Draft_37 Tian_Draft_38 Tian_Draft_39 Tian_Draft_40 Tian_Draft_41 Tian_Draft_42 Tian_Draft_43 Tian_Draft_44 Tian_Draft_45 Tian_Draft_46 Tian_Draft_47 Tian_Draft_48 Tian_Draft_49 Tian_Draft_50 Tian_Draft_51 Tian_Draft_52 Tian_Draft_53 Tian_Draft_54 Tian_Draft_55 Tian_Draft_56 Tian_Draft_57 Tian_Draft_58 Tian_Draft_59 Tian_Draft_60 Tian_Draft_61 Tian_Draft_62 Tian_Draft_63 Tian_Draft_64 Tian_Draft_65 Tian_Draft_66 Tian_Draft_67 Tian_Draft_68 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 154 |
| Pitt Freezer Box Grid# | H8 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
