Arthrobacter phage MiniMommy
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Detailed Information for Phage MiniMommy
Discovery Information
Isolation HostArthrobacter sulfureus ATCC 19098
Former namesMicroMommy
Found ByCindy Kim
Year Found2023
Location FoundLos Angeles, CA US
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates34.06096 N, 118.44316 W Map
Discovery NotesPhage amplification was performed by making an enriched culture. 25 mL of PYCa and 4 mL of 48 hour mixed culture of Arthrobacter was added to the soil sample, which was subsequently incubated at 30ºC for 48 hours. Following filtration by using a 0.22 µm syringe, a 1-plate plaque assay was performed on Arthrobacter species, A. atrocyaneus, A. sulfureus, and A. globiformis to determine if enriched isolate contained phage. Plaques were found to be most prominent on A. sulfureus, which was used as the host bacteria for all subsequent experiments.

Team #1 Name: Daddy Gene Blot
Team #1 Members: Mulin Li, Diana Reyimjan, Sophie Barden, Niels Dawson, Cindy Kim

Team #2 Name: KERMit the Phage
Team #2 Members: Esha Chawla, Madeleine Lim, Ryan Hoang, Kirthana Pisipati

AL Quarter and Lab section: W23-1C
Instructor: Amanda Freise
Teaching Assistant: Juliet Stephenson
Undergraduate Assistant(s): Alexis and Kylie
Naming NotesWe are daddy and we need a mommy.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJul 19, 2023
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage795
Genome length (bp)43680
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGAACTGGCAT
GC Content66.8%
Fasta file available?Yes: Download fasta file
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of Pittsburgh at Greensburg
Annotation StatusBeing Annotated (Expected completion by 10/1/2024)
Plaque NotesHalo, 1mm diameter
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 184
Pitt Freezer Box Grid# E4
Available Files
Plaque PictureDownload