The Actinobacteriophage Database

Arthrobacter phage MiniMommy

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Detailed Information for Phage MiniMommy
Discovery Information
Isolation Host	Arthrobacter sulfureus ATCC 19098
Former names	MicroMommy
Found By	Cindy Kim
Year Found	2023
Location Found	Los Angeles, CA US
Finding Institution	University of California, Los Angeles
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	34.06096 N, 118.44316 W Map
Discovery Notes	Phage amplification was performed by making an enriched culture. 25 mL of PYCa and 4 mL of 48 hour mixed culture of Arthrobacter was added to the soil sample, which was subsequently incubated at 30ºC for 48 hours. Following filtration by using a 0.22 µm syringe, a 1-plate plaque assay was performed on Arthrobacter species, A. atrocyaneus, A. sulfureus, and A. globiformis to determine if enriched isolate contained phage. Plaques were found to be most prominent on A. sulfureus, which was used as the host bacteria for all subsequent experiments. Team #1 Name: Daddy Gene Blot Team #1 Members: Mulin Li, Diana Reyimjan, Sophie Barden, Niels Dawson, Cindy Kim Team #2 Name: KERMit the Phage Team #2 Members: Esha Chawla, Madeleine Lim, Ryan Hoang, Kirthana Pisipati AL Quarter and Lab section: W23-1C Instructor: Amanda Freise Teaching Assistant: Juliet Stephenson Undergraduate Assistant(s): Alexis and Kylie
Naming Notes	We are daddy and we need a mommy.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jul 19, 2023
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina Sequencing
Approximate Shotgun Coverage	795
Genome length (bp)	43680
Character of genome ends	3' Sticky Overhang
Overhang Length	11 bases
Overhang Sequence	CGAACTGGCAT
GC Content	66.8%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	AZ
Subcluster	AZ4
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View AinMach (None) Exile (None) Moss (None) Soondubu (None) Adolin (AZ1) Adumb2043 (AZ1) AEgle (AZ1) AGrandiflora (AZ1) Amyev (AZ1) Asa16 (AZ1) Ascela (AZ1) Berrie (AZ1) Cassia (AZ1) Community (AZ1) Crewmate (AZ1) Cyan (AZ1) DrManhattan (AZ1) DrSierra (AZ1) Elezi (AZ1) Eraser (AZ1) Iter (AZ1) IttyBittyPiggy (AZ1) Janeemi (AZ1) Joemato (AZ1) JohnDoe (AZ1) Jstan (AZ1) JuneStar (AZ1) Kaylissa (AZ1) KeAlii (AZ1) Lego (AZ1) Lizalica (AZ1) London (AZ1) MissSwiss (AZ1) Mudpuppy (AZ1) Niobe (AZ1) Nitro (AZ1) ObiToo (AZ1) Phives (AZ1) Pixelle (AZ1) Powerpuff (AZ1) Pumpkins (AZ1) Reedo (AZ1) Simpson (AZ1) Sue2 (AZ1) Tallboi (AZ1) Tbone (AZ1) TforTroy (AZ1) Tian (AZ1) Tuck (AZ1) Turab (AZ1) Tutumahutu (AZ1) VResidence (AZ1) Warda (AZ1) Wildwest (AZ1) Yang (AZ1) YesChef (AZ1) Liebe (AZ2) MaGuCo (AZ2) Maureen (AZ2) Snek (AZ3) Tweety19 (AZ3) Emotion (AZ4) JasmineDragon (AZ4) MiniMommy (AZ4) ShakeItOph (AZ4) VroomVroom (AZ4)
Annotating Institution	University of Pittsburgh at Greensburg
Annotation Status	Being Annotated (Expected completion by 10/1/2024)
Plaque Notes	Halo, 1mm diameter
Has been Phamerated?	Yes
Gene List	Click to View MiniMommy_Draft_1 MiniMommy_Draft_2 MiniMommy_Draft_3 MiniMommy_Draft_4 MiniMommy_Draft_5 MiniMommy_Draft_6 MiniMommy_Draft_7 MiniMommy_Draft_8 MiniMommy_Draft_9 MiniMommy_Draft_10 MiniMommy_Draft_11 MiniMommy_Draft_12 MiniMommy_Draft_13 MiniMommy_Draft_14 MiniMommy_Draft_15 MiniMommy_Draft_16 MiniMommy_Draft_17 MiniMommy_Draft_18 MiniMommy_Draft_19 MiniMommy_Draft_20 MiniMommy_Draft_21 MiniMommy_Draft_22 MiniMommy_Draft_23 MiniMommy_Draft_24 MiniMommy_Draft_25 MiniMommy_Draft_26 MiniMommy_Draft_27 MiniMommy_Draft_28 MiniMommy_Draft_29 MiniMommy_Draft_30 MiniMommy_Draft_31 MiniMommy_Draft_32 MiniMommy_Draft_33 MiniMommy_Draft_34 MiniMommy_Draft_35 MiniMommy_Draft_36 MiniMommy_Draft_37 MiniMommy_Draft_38 MiniMommy_Draft_39 MiniMommy_Draft_40 MiniMommy_Draft_41 MiniMommy_Draft_42 MiniMommy_Draft_43 MiniMommy_Draft_44 MiniMommy_Draft_45 MiniMommy_Draft_46 MiniMommy_Draft_47 MiniMommy_Draft_48 MiniMommy_Draft_49 MiniMommy_Draft_50 MiniMommy_Draft_51 MiniMommy_Draft_52 MiniMommy_Draft_53 MiniMommy_Draft_54 MiniMommy_Draft_55 MiniMommy_Draft_56 MiniMommy_Draft_57 MiniMommy_Draft_58 MiniMommy_Draft_59 MiniMommy_Draft_60 MiniMommy_Draft_61 MiniMommy_Draft_62 MiniMommy_Draft_63 MiniMommy_Draft_64 MiniMommy_Draft_65 MiniMommy_Draft_66 MiniMommy_Draft_67 MiniMommy_Draft_68 MiniMommy_Draft_69 MiniMommy_Draft_70 MiniMommy_Draft_72 MiniMommy_Draft_73
Publication Info
Uploaded to GenBank?	No
GenBank Accession	None yet
Refseq Number	None yet
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	184
Pitt Freezer Box Grid#	E4
Available Files
Plaque Picture	Download

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