Arthrobacter phage ShakeItOph
ShakeItOph Plaque Picture
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Detailed Information for Phage ShakeItOph
Discovery Information
Isolation HostArthrobacter sulfureus ATCC 19098
Duplicates of this PhageSimonMango
Found ByMrudula Akkinepally
Year Found2023
Location FoundLos Angeles, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates34.067783 N, 118.450455 W Map
Discovery NotesSoil from suburban environment. Environment was not sloped. Sample collected from a mini garden space on a balcony near a busy street at 5cm deep and ambient temperature of 23 deg C.

Host-Enrichment using Arthrobacter sulfureus, Arthrobacter sp., and Arthrobacter globiformis. The soil sample containing the phage was incubated at 30 degrees Celsius for 15 hours with the host bacteria mixture. The mixture was then plated and incubated for 30 degrees Celsius for 48 hours to elicit plaque formation for observation.

AL Quarter and Lab section: F23-1A
Instructor: Dr. Tejas Bouklas
Teaching Assistant: Dom Garza
Undergraduate Assistant(s): Geethika Janga and Saineha Maddineni

Team #1 Name: Plaque to December
Team #1 Members: Hannah Joo, Alessia Giusti, Bella Garcia, and Mrudula Akkinepally
Naming NotesNamed after Taylor Swift's Shake It Off
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 11, 2024
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1357
Genome length (bp)43672
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGAACTGGCAT
GC Content66.8%
Fasta file available?Yes: Download fasta file
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of California, Los Angeles
Annotation StatusOverdue (Expected completion by 5/1/2024)
Plaque NotesBullseye plaque; 20mm in diameter
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 184
Pitt Freezer Box Grid# E10
Available Files
EM PictureDownload