Arthrobacter phage VroomVroom
VroomVroom Plaque Picture
VroomVroom EM Picture
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Detailed Information for Phage VroomVroom
Discovery Information
Isolation HostArthrobacter sulfureus NRRL B-14730
Found ByKevin Olvera
Year Found2022
Location FoundLos Angeles, CA US
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates34.069167 N, 118.451667 W Map
Discovery NotesIsolated by TA Dominic Garza

An enriched culture was prepared by combining 15 mL of soil with 25 mL of PYCA broth. The bacterial strains of A. sp., A. globiformis, A. sulfureus and A. atrocyaneus were then added in a ratio of 1:5:192:588 respectively. The enriched culture was incubated at 30℃ for 48 hours with shaking. After filtering with a 0.22 µm filter, the sample was plated on PYCA plates with 2X PYCa soft agar using the standard pour plate method.
Naming NotesThe name is a direct reference to the EP Vroom Vroom – produced by singer/songwriter Charli XCX.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 8, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1698
Genome length (bp)43230
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGAACTGGCAT
GC Content66.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterAZ
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of California, Los Angeles
Annotation StatusAwaiting_QC
Plaque NotesTurbid with fuzzy edges
diameter: 1.5 mm
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 156
Pitt Freezer Box Grid# A2