Arthrobacter phage Nitro
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Detailed Information for Phage Nitro
Discovery Information
Isolation HostArthrobacter globiformis B-2979
Found ByJason Summerfield
Year Found2020
Location FoundNew Haven, CT United States of America
Finding InstitutionSouthern Connecticut State University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates41.334167 N, 72.945556 W Map
Discovery NotesThe temperature was around 17 degrees Celsius on the date of discovery 8-31-2020. The soil was dug 2 cm deep and 15 mL of soil was collected. It was found next to a tree and beside a building. The day prior was stormy weather making the soil mud-like.
Naming NotesNamed after my dog who recently passed before starting the SEA PHAGES program. He passed in an unfortunate circumstance and his name was Nitro. To honor him, I am naming my phage after him.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 26, 2021
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage25
Genome length (bp)44379
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGAAGGGGCAT
GC Content66.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterAZ
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionWashington State University
Annotation StatusBeing Annotated (Expected completion by 5/1/2021)
Plaque NotesSmall to medium sized plaques. Temperate phage. Titer is 7.1e10. Phage has not yet been sequenced. Results of TEM have yet to be received.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 7.1e10
Available Files
Plaque PictureDownload