Arthrobacter phage Powerpuff
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Detailed Information for Phage Powerpuff
Discovery Information
Isolation HostArthrobacter globiformis B-2979
Found ByHilory Foster
Year Found2019
Location FoundValencia, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature25°C
GPS Coordinates34.443624 N, 118.609545 W Map
Discovery NotesThe sample was collected by Hilory Foster in Los Angeles, CA. Arthrobacter phage Powerpuff was isolated from soil by enrichment plating at 25°C and was characterized and annotated by Pauline E. Aghamalian, Anya S. Alag, Erika M. Capehart, Hilory Foster, Daisy Hernandez-Casas, Sophia C. Wang, Heather A. Angel, Abbas Hakim, and Derek Q. Lam.

Instructor: Amanda Freise
Teaching Assistants: Ryan Ngo, Andrew Kapinos
Undergraduate Assistants: Yvette Lakkis, Andrew Lee
Naming NotesIt was named after the Powerpuff girls because they were powerful and this phage is also powerful by being able to lyse through a whole plate.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 21, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage705
Genome length (bp)44651
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGAAGGGGCAT
GC Content67.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterAZ
Subcluster--
Cluster Life CycleTemperate
Lysogeny NotesDifferent conditions have produced either bullseye or completely turbid plaques and, in a few cases, clear plaques have been observed. The switch from bullseye plaques to turbid/clear plaques indicate a switch in life cycle which is indicative of the temperate life cycle.

Serine integrase was identified during annotation.
Other Cluster Members
Annotating InstitutionUniversity of California, Los Angeles
Annotation StatusIn GenBank
Plaque Notesturbid and bullseye
Average diameter: 1mm
MorphotypeSiphoviridae
Number of Genes71
Has been Phamerated?Yes
Gene List

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Powerpuff_1
(terminase small subunit)

Powerpuff_2
(terminase large subunit)

Powerpuff_3
(portal protein)

Powerpuff_4
(VIP2-like ADP-ribosyltransferase toxin)

Powerpuff_5

Powerpuff_6
(scaffolding protein)

Powerpuff_7
(major capsid protein)

Powerpuff_8
(head-to-tail adaptor)

Powerpuff_9

Powerpuff_10
(head-to-tail stopper)

Powerpuff_11

Powerpuff_12
(tail terminator)

Powerpuff_13
(major tail protein)

Powerpuff_14
(tail assembly chaperone)

Powerpuff_15
(tail assembly chaperone)

Powerpuff_16
(tape measure protein)

Powerpuff_17
(minor tail protein)

Powerpuff_18
(minor tail protein)

Powerpuff_19
(minor tail protein)

Powerpuff_20
(minor tail protein)

Powerpuff_21

Powerpuff_22

Powerpuff_23

Powerpuff_24
(deoxynucleoside monophosphate kinase)

Powerpuff_25

Powerpuff_26
(exonuclease)

Powerpuff_27
(nucleoside deoxyribosyltransferase)

Powerpuff_28
(LAGLIDADG endonuclease)

Powerpuff_29

Powerpuff_30

Powerpuff_31

Powerpuff_32

Powerpuff_33

Powerpuff_34

Powerpuff_35
(NrdH-like glutaredoxin)

Powerpuff_36

Powerpuff_37
(holliday junction resolvase)

Powerpuff_38

Powerpuff_39
(DNA methylase)

Powerpuff_40
(DNA primase/helicase)

Powerpuff_41

Powerpuff_42

Powerpuff_43
(DNA polymerase I)

Powerpuff_44

Powerpuff_45
(DNA ligase)

Powerpuff_46

Powerpuff_47
(DNA binding protein)

Powerpuff_48
(helix-turn-helix DNA-binding protein)

Powerpuff_49
(SprT-like protease)

Powerpuff_50

Powerpuff_51

Powerpuff_52
(serine integrase)

Powerpuff_53

Powerpuff_54

Powerpuff_55

Powerpuff_56

Powerpuff_57

Powerpuff_58

Powerpuff_59
(endolysin)

Powerpuff_60

Powerpuff_61

Powerpuff_62

Powerpuff_63

Powerpuff_64

Powerpuff_65

Powerpuff_66

Powerpuff_67

Powerpuff_68

Powerpuff_69
(HNH endonuclease)

Powerpuff_70

Powerpuff_71
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMN703413
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload