| Detailed Information for Phage Powerpuff | |
| Discovery Information | |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Hilory Foster |
| Year Found | 2019 |
| Location Found | Valencia, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 25°C |
| GPS Coordinates | 34.443624 N, 118.609545 W Map |
| Discovery Notes | The sample was collected by Hilory Foster in Los Angeles, CA. Arthrobacter phage Powerpuff was isolated from soil by enrichment plating at 25°C and was characterized and annotated by Pauline E. Aghamalian, Anya S. Alag, Erika M. Capehart, Hilory Foster, Daisy Hernandez-Casas, Sophia C. Wang, Heather A. Angel, Abbas Hakim, and Derek Q. Lam. Instructor: Amanda Freise Teaching Assistants: Ryan Ngo, Andrew Kapinos Undergraduate Assistants: Yvette Lakkis, Andrew Lee |
| Naming Notes | It was named after the Powerpuff girls because they were powerful and this phage is also powerful by being able to lyse through a whole plate. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 21, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 705 |
| Genome length (bp) | 44651 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAAGGGGCAT |
| GC Content | 67.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AZ |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Different conditions have produced either bullseye or completely turbid plaques and, in a few cases, clear plaques have been observed. The switch from bullseye plaques to turbid/clear plaques indicate a switch in life cycle which is indicative of the temperate life cycle. Serine integrase was identified during annotation. |
| Other Cluster Members | |
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | In GenBank |
| Plaque Notes | turbid and bullseye Average diameter: 1mm |
| Morphotype | Siphoviridae |
| Number of Genes | 71 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewPowerpuff_1 Powerpuff_2 Powerpuff_3 Powerpuff_4 Powerpuff_5 Powerpuff_6 Powerpuff_7 Powerpuff_8 Powerpuff_9 Powerpuff_10 Powerpuff_11 Powerpuff_12 Powerpuff_13 Powerpuff_14 Powerpuff_15 Powerpuff_16 Powerpuff_17 Powerpuff_18 Powerpuff_19 Powerpuff_20 Powerpuff_21 Powerpuff_22 Powerpuff_23 Powerpuff_24 Powerpuff_25 Powerpuff_26 Powerpuff_27 Powerpuff_28 Powerpuff_29 Powerpuff_30 Powerpuff_31 Powerpuff_32 Powerpuff_33 Powerpuff_34 Powerpuff_35 Powerpuff_36 Powerpuff_37 Powerpuff_38 Powerpuff_39 Powerpuff_40 Powerpuff_41 Powerpuff_42 Powerpuff_43 Powerpuff_44 Powerpuff_45 Powerpuff_46 Powerpuff_47 Powerpuff_48 Powerpuff_49 Powerpuff_50 Powerpuff_51 Powerpuff_52 Powerpuff_53 Powerpuff_54 Powerpuff_55 Powerpuff_56 Powerpuff_57 Powerpuff_58 Powerpuff_59 Powerpuff_60 Powerpuff_61 Powerpuff_62 Powerpuff_63 Powerpuff_64 Powerpuff_65 Powerpuff_66 Powerpuff_67 Powerpuff_68 Powerpuff_69 Powerpuff_70 Powerpuff_71 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN703413 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |