| Discovery Information |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Michelle Zorawik |
| Year Found | 2021 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.056805 N, 118.446412 W Map |
| Discovery Notes | The soil was collected from a fenced-in front lawn of an apartment complex located in an urban setting. It was covered with decaying leaves, and was surrounded by Bird of Paradise plants and rose bushes. The soil was saturated as the site is watered with sprinklers daily.
Depth from which sample was taken: 5 cm
Air temperature: 16 degrees C
Isolated in 103BL-F21-1B
Instructor: Amanda Freise; Ana Garcia Vedrenne
Teaching Assistant: Michelle Zorawik
Undergraduate Assistant(s): Himani Parikh
Team Name: Baby Come Plaque
Team Members: Noah Luke Picart Canio, Malika Jakupova, Kathryn Elizabeth Howe, Ninette Darlin Linares Cardona, Alliya Haeri |
| Naming Notes | The phage was named Tutumahutu to honor and preserve an imaginary character invented with my closest and longest friend during our childhood. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 26, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 667 |
| Genome length (bp) | 43693 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAAGGGGCAT |
| GC Content | 67.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AZ |
| Subcluster | AZ1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques primarily were turbid and round, with fuzzy margins (3-4 mm in diameter). Final plaque assays showed smaller (~2mm diameter), round and clear plaques. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP978774 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
139 |
| Pitt Freezer Box Grid# |
G12 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
