Arthrobacter phage IttyBittyPiggy
Know something about this phage that we don't? Modify its data.
Detailed Information for Phage IttyBittyPiggy
Discovery Information
Isolation HostArthrobacter atrocyaneus NRRL B-2883
Found BySamantha Padilla
Year Found2023
Location FoundPacific Palisades, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates34.04197 N, 118.5554 W Map
Discovery NotesIsolated by: Dominic Garza, Michelle Zorawik

An enriched culture was prepared by combining 15 mL of soil with 25 mL of PYCA broth. The bacterial strains of A. sp., A. globiformis, A. sulfureus and A. atrocyaneus were then added in equal concentrations. The enriched culture was incubated at 30℃ for 48 hours with shaking. After filtering with a 0.22 µm filter, the sample was plated on PYCA plates with 2X PYCa soft agar using the standard pour plate method.
Naming NotesThe name is a direct reference to a song on Nicki Minaj’s 2009 mixtape titled ‘Beam Me Up Scotty’ which was reissued in 2021.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedOct 30, 2023
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1280
Genome length (bp)42206
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGAACCGGCAT
GC Content68.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterAZ
SubclusterAZ1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of California, Los Angeles
Annotation StatusOverdue (Expected completion by 6/14/2024)
Plaque NotesDescription of plaque morphology (e.g., halos, clear or turbid): Halo with a clear center and turbid margins.

Average size (and size range) diameter in mm: 1-1.5 mm
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

IttyBittyPiggy_Draft_1

IttyBittyPiggy_Draft_2

IttyBittyPiggy_Draft_3

IttyBittyPiggy_Draft_4

IttyBittyPiggy_Draft_5

IttyBittyPiggy_Draft_6

IttyBittyPiggy_Draft_7

IttyBittyPiggy_Draft_8

IttyBittyPiggy_Draft_9

IttyBittyPiggy_Draft_10

IttyBittyPiggy_Draft_11

IttyBittyPiggy_Draft_12

IttyBittyPiggy_Draft_13

IttyBittyPiggy_Draft_14

IttyBittyPiggy_Draft_15

IttyBittyPiggy_Draft_16

IttyBittyPiggy_Draft_17

IttyBittyPiggy_Draft_18

IttyBittyPiggy_Draft_19

IttyBittyPiggy_Draft_20

IttyBittyPiggy_Draft_21

IttyBittyPiggy_Draft_22

IttyBittyPiggy_Draft_23

IttyBittyPiggy_Draft_24

IttyBittyPiggy_Draft_25

IttyBittyPiggy_Draft_26

IttyBittyPiggy_Draft_27

IttyBittyPiggy_Draft_28

IttyBittyPiggy_Draft_29

IttyBittyPiggy_Draft_30

IttyBittyPiggy_Draft_31

IttyBittyPiggy_Draft_32

IttyBittyPiggy_Draft_33

IttyBittyPiggy_Draft_34

IttyBittyPiggy_Draft_35

IttyBittyPiggy_Draft_36

IttyBittyPiggy_Draft_37

IttyBittyPiggy_Draft_38

IttyBittyPiggy_Draft_39

IttyBittyPiggy_Draft_40

IttyBittyPiggy_Draft_41

IttyBittyPiggy_Draft_42

IttyBittyPiggy_Draft_43

IttyBittyPiggy_Draft_44

IttyBittyPiggy_Draft_45

IttyBittyPiggy_Draft_46

IttyBittyPiggy_Draft_47

IttyBittyPiggy_Draft_48

IttyBittyPiggy_Draft_49

IttyBittyPiggy_Draft_50

IttyBittyPiggy_Draft_51

IttyBittyPiggy_Draft_52

IttyBittyPiggy_Draft_53

IttyBittyPiggy_Draft_54

IttyBittyPiggy_Draft_55

IttyBittyPiggy_Draft_56

IttyBittyPiggy_Draft_57

IttyBittyPiggy_Draft_58

IttyBittyPiggy_Draft_59

IttyBittyPiggy_Draft_60

IttyBittyPiggy_Draft_61

IttyBittyPiggy_Draft_62

IttyBittyPiggy_Draft_63

IttyBittyPiggy_Draft_64

IttyBittyPiggy_Draft_65

IttyBittyPiggy_Draft_66

IttyBittyPiggy_Draft_67

IttyBittyPiggy_Draft_68

IttyBittyPiggy_Draft_69

Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 184
Pitt Freezer Box Grid# D12
Available Files
Plaque PictureDownload