| Detailed Information for Phage IttyBittyPiggy |
| Discovery Information |
| Isolation Host | Arthrobacter atrocyaneus NRRL B-2883 |
| Found By | Samantha Padilla |
| Year Found | 2023 |
| Location Found | Pacific Palisades, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.04197 N, 118.5554 W Map |
| Discovery Notes | Isolated by: Dominic Garza, Michelle Zorawik
An enriched culture was prepared by combining 15 mL of soil with 25 mL of PYCA broth. The bacterial strains of A. sp., A. globiformis, A. sulfureus and A. atrocyaneus were then added in equal concentrations. The enriched culture was incubated at 30℃ for 48 hours with shaking. After filtering with a 0.22 µm filter, the sample was plated on PYCA plates with 2X PYCa soft agar using the standard pour plate method. |
| Naming Notes | The name is a direct reference to a song on Nicki Minaj’s 2009 mixtape titled ‘Beam Me Up Scotty’ which was reissued in 2021. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Oct 30, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1280 |
| Genome length (bp) | 42206 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAACCGGCAT |
| GC Content | 68.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AZ |
| Subcluster | AZ1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | Awaiting_QC |
| Plaque Notes | Description of plaque morphology (e.g., halos, clear or turbid): Halo with a clear center and turbid margins.
Average size (and size range) diameter in mm: 1-1.5 mm |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
184 |
| Pitt Freezer Box Grid# |
D12 |
| Available Files |
| Plaque Picture | Download |
