Arthrobacter phage IttyBittyPiggy
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Detailed Information for Phage IttyBittyPiggy
Discovery Information
Isolation HostArthrobacter atrocyaneus NRRL B-2883
Found BySamantha Padilla
Year Found2023
Location FoundPacific Palisades, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates34.04197 N, 118.5554 W Map
Discovery NotesIsolated by: Dominic Garza, Michelle Zorawik

An enriched culture was prepared by combining 15 mL of soil with 25 mL of PYCA broth. The bacterial strains of A. sp., A. globiformis, A. sulfureus and A. atrocyaneus were then added in equal concentrations. The enriched culture was incubated at 30℃ for 48 hours with shaking. After filtering with a 0.22 µm filter, the sample was plated on PYCA plates with 2X PYCa soft agar using the standard pour plate method.
Naming NotesThe name is a direct reference to a song on Nicki Minaj’s 2009 mixtape titled ‘Beam Me Up Scotty’ which was reissued in 2021.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedOct 30, 2023
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1280
Genome length (bp)42206
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGAACCGGCAT
GC Content68.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterAZ
SubclusterAZ1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of California, Los Angeles
Annotation StatusAwaiting_QC
Plaque NotesDescription of plaque morphology (e.g., halos, clear or turbid): Halo with a clear center and turbid margins.

Average size (and size range) diameter in mm: 1-1.5 mm
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 184
Pitt Freezer Box Grid# D12
Available Files
Plaque PictureDownload