| Detailed Information for Phage MissSwiss | |
| Discovery Information | |
| Isolation Host | Arthrobacter atrocyaneus NRRL B-2883 |
| Found By | Cristal Santaolaya |
| Year Found | 2023 |
| Location Found | Shadow Hills, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.2728 N, 118.33717 W Map |
| Discovery Notes | Moist and slightly saturated soil that contained some small ricks, shrubbery, and grass. Near a small stream that flowed down from a mountain. The surroundings were rocky and had lots of vegetation. Collection site was near a highway and even flowed beneath a freeway bridge where the ground was flat. AL Quarter and Lab section (ie. F19-1A): S23-1A Instructor: Dr. Tejas Bouklas Teaching Assistant: Dominic Garza Undergraduate Assistant(s): Samantha Padilla, Trinity Vu Team #1 Name: Phantom Phages Team #1 Members: Elisha Anne Santos, Emma Yang, Kaley Qin, Cristal Santaolaya |
| Naming Notes | The plaques formed by the phage are clear, circular, and with sharp edges. When there are multiple PFU on a bacterial lawn, everything looks like a piece of swiss cheese. Thus, we named her MissSwiss. Also, all of the members on this team really like cheese. :) |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Sep 6, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2756 |
| Genome length (bp) | 42615 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAAGAGGCAT |
| End Determination Notes | at position 15912 bp 50% of bases contain an A to C at substitution |
| GC Content | 66.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AZ |
| Subcluster | AZ1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to ViewAinMach (None) Exile (None) Moss (None) Soondubu (None) Adolin (AZ1) Adumb2043 (AZ1) AEgle (AZ1) AGrandiflora (AZ1) Amyev (AZ1) Asa16 (AZ1) Ascela (AZ1) Berrie (AZ1) Cassia (AZ1) Community (AZ1) Crewmate (AZ1) Cyan (AZ1) DrManhattan (AZ1) DrSierra (AZ1) Elezi (AZ1) Eraser (AZ1) Iter (AZ1) IttyBittyPiggy (AZ1) Janeemi (AZ1) Joemato (AZ1) JohnDoe (AZ1) Jstan (AZ1) JuneStar (AZ1) Kaylissa (AZ1) KeAlii (AZ1) Lego (AZ1) Lizalica (AZ1) London (AZ1) MissSwiss (AZ1) Mudpuppy (AZ1) Niobe (AZ1) Nitro (AZ1) ObiToo (AZ1) Phives (AZ1) Pixelle (AZ1) Powerpuff (AZ1) Pumpkins (AZ1) Reedo (AZ1) Simpson (AZ1) Sue2 (AZ1) Tallboi (AZ1) Tbone (AZ1) TforTroy (AZ1) Tian (AZ1) Tuck (AZ1) Turab (AZ1) Tutumahutu (AZ1) VResidence (AZ1) Warda (AZ1) Wildwest (AZ1) Yang (AZ1) YesChef (AZ1) Liebe (AZ2) MaGuCo (AZ2) Maureen (AZ2) Snek (AZ3) Tweety19 (AZ3) Emotion (AZ4) JasmineDragon (AZ4) MiniMommy (AZ4) ShakeItOph (AZ4) VroomVroom (AZ4) |
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | Awaiting_QC |
| Plaque Notes | Description of plaque morphology (e.g., halos, clear or turbid): Circular and clear with sharp edged margins Average size (and size range) diameter in mm: Range is between 1-2 mm in diameter |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewMissSwiss_Draft_1 MissSwiss_Draft_2 MissSwiss_Draft_3 MissSwiss_Draft_4 MissSwiss_Draft_5 MissSwiss_Draft_6 MissSwiss_Draft_7 MissSwiss_Draft_8 MissSwiss_Draft_9 MissSwiss_Draft_10 MissSwiss_Draft_11 MissSwiss_Draft_12 MissSwiss_Draft_13 MissSwiss_Draft_14 MissSwiss_Draft_15 MissSwiss_Draft_16 MissSwiss_Draft_17 MissSwiss_Draft_18 MissSwiss_Draft_19 MissSwiss_Draft_20 MissSwiss_Draft_21 MissSwiss_Draft_22 MissSwiss_Draft_23 MissSwiss_Draft_24 MissSwiss_Draft_25 MissSwiss_Draft_26 MissSwiss_Draft_27 MissSwiss_Draft_28 MissSwiss_Draft_29 MissSwiss_Draft_30 MissSwiss_Draft_31 MissSwiss_Draft_32 MissSwiss_Draft_33 MissSwiss_Draft_34 MissSwiss_Draft_35 MissSwiss_Draft_36 MissSwiss_Draft_37 MissSwiss_Draft_38 MissSwiss_Draft_39 MissSwiss_Draft_40 MissSwiss_Draft_41 MissSwiss_Draft_42 MissSwiss_Draft_43 MissSwiss_Draft_44 MissSwiss_Draft_45 MissSwiss_Draft_46 MissSwiss_Draft_47 MissSwiss_Draft_48 MissSwiss_Draft_49 MissSwiss_Draft_50 MissSwiss_Draft_51 MissSwiss_Draft_52 MissSwiss_Draft_53 MissSwiss_Draft_54 MissSwiss_Draft_55 MissSwiss_Draft_56 MissSwiss_Draft_57 MissSwiss_Draft_58 MissSwiss_Draft_59 MissSwiss_Draft_60 MissSwiss_Draft_61 MissSwiss_Draft_62 MissSwiss_Draft_63 MissSwiss_Draft_64 MissSwiss_Draft_65 MissSwiss_Draft_66 MissSwiss_Draft_67 MissSwiss_Draft_68 MissSwiss_Draft_69 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 184 |
| Pitt Freezer Box Grid# | E5 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
