| Detailed Information for Phage YesChef | |
| Discovery Information | |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Edward Lee |
| Year Found | 2019 |
| Location Found | Los Angeles, CA, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.052707 N, 118.44657 W Map |
| Discovery Notes | Arthrobacter phage YesChef was isolated by Edward Lee from soil collected near trees, tall grasses, next to a playground, and municipal site, using direct plating at 30C. The phage was characterized by Team Can’t Phage Us (Edward Lee, Aaron Bernardino, Stephanie Demo). Instructor: Amanda Freise Teaching Assistant: Andrew Kapinos Undergraduate Assistants: Emily Pham and Oscar Lim |
| Naming Notes | Whenever our TA told us to do something, we started replying with “yes chef” and “thank you chef” like in Hell’s Kitchen/Masterchef. We respect our TA like he is the Gordon Ramsay of virology. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 3, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2419 |
| Genome length (bp) | 43510 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAAGGGGCAT |
| GC Content | 67.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AZ |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | In GenBank |
| Plaque Notes | Description of plaque morphology: bullseye Average size (and size range) diameter in mm: 4mm |
| Morphotype | Siphoviridae |
| Number of Genes | 69 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewYesChef_1 YesChef_2 YesChef_3 YesChef_4 YesChef_5 YesChef_6 YesChef_7 YesChef_8 YesChef_9 YesChef_10 YesChef_11 YesChef_12 YesChef_13 YesChef_14 YesChef_15 YesChef_16 YesChef_17 YesChef_18 YesChef_19 YesChef_20 YesChef_21 YesChef_22 YesChef_23 YesChef_24 YesChef_25 YesChef_26 YesChef_27 YesChef_28 YesChef_29 YesChef_30 YesChef_31 YesChef_32 YesChef_33 YesChef_34 YesChef_35 YesChef_36 YesChef_37 YesChef_38 YesChef_39 YesChef_40 YesChef_41 YesChef_42 YesChef_43 YesChef_44 YesChef_45 YesChef_46 YesChef_47 YesChef_48 YesChef_49 YesChef_50 YesChef_51 YesChef_52 YesChef_53 YesChef_54 YesChef_55 YesChef_56 YesChef_57 YesChef_58 YesChef_59 YesChef_60 YesChef_61 YesChef_62 YesChef_63 YesChef_64 YesChef_65 YesChef_66 YesChef_67 YesChef_68 YesChef_69 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT024871 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 116 |
| Pitt Freezer Box Grid# | D3 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
