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Arthrobacter phage YesChef
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Detailed Information for Phage YesChef
Discovery Information
Isolation HostArthrobacter globiformis B-2979
Found ByEdward Lee
Year Found2019
Location FoundLos Angeles, CA, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates34.052707 N, 118.44657 W Map
Discovery NotesArthrobacter phage YesChef was isolated by Edward Lee from soil collected near trees, tall grasses, next to a playground, and municipal site, using direct plating at 30C. The phage was characterized by Team Can’t Phage Us (Edward Lee, Aaron Bernardino, Stephanie Demo).

Instructor: Amanda Freise
Teaching Assistant: Andrew Kapinos
Undergraduate Assistants: Emily Pham and Oscar Lim
Naming NotesWhenever our TA told us to do something, we started replying with “yes chef” and “thank you chef” like in Hell’s Kitchen/Masterchef. We respect our TA like he is the Gordon Ramsay of virology.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 3, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2419
Genome length (bp)43510
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGAAGGGGCAT
GC Content67.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterAZ
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of California, Los Angeles
Annotation StatusIn GenBank
Plaque NotesDescription of plaque morphology: bullseye

Average size (and size range) diameter in mm: 4mm
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

YesChef_1
(terminase small subunit)

YesChef_2
(terminase large subunit)

YesChef_3
(portal protein)

YesChef_4
(ADP-ribosyltransferase domain and MuF-like fusion protein)

YesChef_5

YesChef_6
(scaffolding protein)

YesChef_7
(major capsid protein)

YesChef_8
(head-to-tail adaptor)

YesChef_9

YesChef_10
(head-to-tail stopper)

YesChef_11

YesChef_12
(tail terminator)

YesChef_13
(major tail protein)

YesChef_14
(tail assembly chaperone)

YesChef_15
(tail assembly chaperone)

YesChef_16
(tape measure protein)

YesChef_17
(minor tail protein)

YesChef_18
(minor tail protein)

YesChef_19
(minor tail protein)

YesChef_20
(minor tail protein)

YesChef_21

YesChef_22

YesChef_23

YesChef_24
(adenylate kinase)

YesChef_25

YesChef_26
(Cas4 family exonuclease)

YesChef_27
(nucleoside deoxyribosyltransferase)

YesChef_28
(LAGLIDADG endonuclease)

YesChef_29

YesChef_30

YesChef_31

YesChef_32

YesChef_33
(NrdH-like glutaredoxin)

YesChef_34

YesChef_35
(holliday junction resolvase)

YesChef_36

YesChef_37
(DNA methylase)

YesChef_38
(DNA primase/helicase)

YesChef_39

YesChef_40

YesChef_41
(DNA polymerase I)

YesChef_42

YesChef_43
(DNA ligase)

YesChef_44

YesChef_45
(RNA polymerase sigma factor)

YesChef_46
(helix-turn-helix DNA binding domain protein)

YesChef_47
(SprT-like protease)

YesChef_48

YesChef_49

YesChef_50
(serine integrase)

YesChef_51

YesChef_52

YesChef_53

YesChef_54

YesChef_55

YesChef_56

YesChef_57
(lysin A, protease M23 domain)

YesChef_58

YesChef_59

YesChef_60

YesChef_61

YesChef_62

YesChef_63

YesChef_64

YesChef_65

YesChef_66

YesChef_67
(HNH endonuclease)

YesChef_68

YesChef_69
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT024871
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload