Arthrobacter phage Pumpkins
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Detailed Information for Phage Pumpkins
Discovery Information
Isolation HostArthrobacter globiformis B-2979
Found ByAllison Hughes
Year Found2023
Location FoundBismarck, ND United States
Finding InstitutionUniversity of Mary
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature26°C
GPS Coordinates46.72378 N, 100.75427 W Map
Discovery NotesThis was found under some dead flower vegetation, and the soil was moist and dark brown with dead vegetation in it.
Naming NotesI chose the name Pumpkin because of my grandma’s pumpkin farm and all the time I have spent there helping out and digging in the fields.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 27, 2023
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2667
Genome length (bp)43976
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGAACCGGCAT
GC Content68.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterAZ
SubclusterAZ1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of Mary
Annotation StatusQC Complete
Plaque NotesIt was a large clear plaque, with a halo of 2 mm, with an average size 13mm for the phages.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Pumpkins_1
(terminase, small subunit)

Pumpkins_2
(terminase, large subunit)

Pumpkins_3
(portal protein)

Pumpkins_4
(Capsid maturation protease and VIP2-like ADP-ribosyltransferase toxin)

Pumpkins_5

Pumpkins_6
(scaffolding protein)

Pumpkins_7
(major capsid protein)

Pumpkins_8
(head-to-tail adaptor)

Pumpkins_9

Pumpkins_10
(head-to-tail stopper)

Pumpkins_11

Pumpkins_12
(tail terminator)

Pumpkins_13
(major tail protein)

Pumpkins_14
(tail assembly chaperone)

Pumpkins_15
(tail assembly chaperone)

Pumpkins_16
(tape measure protein)

Pumpkins_17
(minor tail protein)

Pumpkins_18
(minor tail protein)

Pumpkins_19
(minor tail protein)

Pumpkins_20
(minor tail protein)

Pumpkins_21

Pumpkins_22

Pumpkins_23

Pumpkins_24
(deoxynucleoside monophosphate kinase)

Pumpkins_25

Pumpkins_26
(exonuclease)

Pumpkins_27

Pumpkins_28
(nucleoside deoxyribosyltransferase)

Pumpkins_29
(LAGLIDADG endonuclease)

Pumpkins_30
(recombination directionality factor)

Pumpkins_31

Pumpkins_32

Pumpkins_33
(NrdH-like glutaredoxin)

Pumpkins_34

Pumpkins_35

Pumpkins_36
(Holliday junction resolvase)

Pumpkins_37
(DNA primase/helicase)

Pumpkins_38

Pumpkins_39

Pumpkins_40
(DNA polymerase I)

Pumpkins_41

Pumpkins_42
(DNA ligase)

Pumpkins_43

Pumpkins_44
(DNA binding protein)

Pumpkins_45
(helix-turn-helix DNA binding domain)

Pumpkins_46
(DNA Methyltransferase)

Pumpkins_47
(SprT-like protease)

Pumpkins_48
(serine integrase)

Pumpkins_49

Pumpkins_50
(RNA binding protein)

Pumpkins_51
(RNA binding protein)

Pumpkins_52

Pumpkins_53

Pumpkins_54

Pumpkins_55

Pumpkins_56
(endolysin)

Pumpkins_57

Pumpkins_58

Pumpkins_59

Pumpkins_60

Pumpkins_61

Pumpkins_62

Pumpkins_63

Pumpkins_64

Pumpkins_65

Pumpkins_66

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 169
Pitt Freezer Box Grid# E4
Available Files
GenBank File for PhameratorDownload