| Detailed Information for Phage Amethyst |
| Discovery Information |
| Isolation Host | Streptomyces xanthochromogenes NRRL B-5410 |
| Found By | Elizabeth Spangler |
| Year Found | 2016 |
| Location Found | Denton, TX USA |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 33.2125 N, 97.14722 W Map |
| Discovery Notes | Soil found in Denton, TX; Temperature was 85° F at soil sample collection. The date and time of soil sample collection was 9/9/16 at 11:08am; the soil collected was dry, loose soil.
The MTL of this phage is 1.2x10^8 pfu/mL; the average diameter of plaques is 2mm; the DNA concentration is 131.8 and the DNA/RNA ratio is 1.93.
In a host range test with the bacteria S. venezuelae, S. albus, S. virginiae, S. griseus, and S. azureus, no plaques were formed using Amethyst. |
| Naming Notes | The name for this phage came from the February birthstone, Amethyst. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 30, 2017 |
| Sequencing Facility | University of North Texas |
| Shotgun Sequencing Method | Illumina |
| Genome length (bp) | 49372 |
| Character of genome ends | Unknown |
| Overhang Length | 11 bases |
| Overhang Sequence | CGCCACGTCTT |
| GC Content | 67.2% |
| Sequencing Notes | at position 19553 approximately 33% of reads contain a G instead of consensus A |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BD |
| Subcluster | BD2 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Titer tests concluded that Amethyst isn't a lysogen. |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 79 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF766044 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
6.0 x 10^8 |
| Pitt Freezer Box# |
36 |
| Pitt Freezer Box Grid# |
C9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
