Streptomyces phage Rusticus
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Detailed Information for Phage Rusticus
Discovery Information
Isolation HostStreptomyces tricolor NRRL B-16925
Found ByRusty Hartman
Year Found2017
Location FoundDenton, TX USA
Finding InstitutionUniversity of North Texas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates33.213763 N, 97.163042 W Map
Discovery NotesThe soil was collected on October 19th 2017 at 9:13 am. The temperature was 54 degrees Fahrenheit. The sample was moist, muddy, and clumpy.
Naming NotesThe name of this originated from my name. I am also a student who is taking Latin, so I chose to turn it into a Latin form. I later realized that the name in Latin actually meant rural, simple, rough, or clownish.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 16, 2019
Sequencing FacilityUniversity of North Texas
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage97
Genome length (bp)50066
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGGGCAGTGAT
GC Content65.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBD
SubclusterBD1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of North Texas
Annotation StatusIn GenBank
Plaque NotesThe plaques are about 1.5 millimeters in diameter. The characteristics are clear and round.
Has been Phamerated?Yes
Gene List

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Rusticus_1

Rusticus_2
(HNH endonuclease)

Rusticus_3

Rusticus_4

Rusticus_5
(thioredoxin)

Rusticus_6
(terminase small subunit)

Rusticus_7
(terminase large subunit)

Rusticus_8
(portal protein)

Rusticus_9
(capsid maturation protease and MuF-like fusion protein)

Rusticus_10
(scaffolding protein)

Rusticus_11
(major capsid protein)

Rusticus_12
(head-to-tail adaptor)

Rusticus_13
(head-to-tail adaptor)

Rusticus_14

Rusticus_15
(head-to-tail adaptor)

Rusticus_16
(major tail protein)

Rusticus_17
(tail assembly chaperone)

Rusticus_18
(tail assembly chaperone)

Rusticus_19
(tape measure protein)

Rusticus_20
(minor tail protein)

Rusticus_21
(minor tail protein)

Rusticus_22

Rusticus_23

Rusticus_24

Rusticus_25

Rusticus_26

Rusticus_27
(LysM-like endolysin)

Rusticus_28

Rusticus_29

Rusticus_30
(immunity repressor)

Rusticus_31

Rusticus_32
(exonuclease)

Rusticus_33

Rusticus_34

Rusticus_35
(deoxycytidylate deaminase)

Rusticus_36
(DnaB-like dsDNA helicase)

Rusticus_37
(holliday junction resolvase)

Rusticus_38

Rusticus_39
(DNA primase)

Rusticus_40
(DNA primase)

Rusticus_41

Rusticus_42

Rusticus_43

Rusticus_44
(exonuclease)

Rusticus_45

Rusticus_46
(helix-turn-helix DNA binding domain protein)

Rusticus_47

Rusticus_48
(ribonucleotide reductase)

Rusticus_49
(Lsr2-like DNA bridging protein)

Rusticus_50
(serine integrase)

Rusticus_51

Rusticus_52
(ThyX-like thymidylate synthase)

Rusticus_53

Rusticus_54

Rusticus_55
(DnaE-like DNA polymerase III)

Rusticus_56

Rusticus_57

Rusticus_58

Rusticus_59
(deoxynucleoside monophosphate kinase)

Rusticus_60

Rusticus_61

Rusticus_62

Rusticus_63

Rusticus_64

Rusticus_65

Rusticus_66
(HNH endonuclease)

Rusticus_67

Rusticus_68

Rusticus_69

Rusticus_70

Rusticus_71

Rusticus_72

Rusticus_73

Rusticus_74

Rusticus_75
(acetyltransferase)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMN096381
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2x10^7
Pitt Freezer Box# 70
Pitt Freezer Box Grid# D8
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload