Streptomyces phage BroPlease
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Detailed Information for Phage BroPlease
Discovery Information
Isolation HostStreptomyces griseus ATCC 10137
Found ByFaith LaBry & Ahmad Sulaiman
Year Found2021
Location FoundDenton, TX United States
Finding InstitutionUniversity of North Texas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates33.254578 N, 97.146434 W Map
Discovery Notes-Obtained from my TA's backyard
-Dug one inch under a grassy surface
-Partially cloudy outside
Naming NotesMy name came from the fact that I had numerous struggles with obtaining and isolating the phage. Both of my soil samples yielded no sign of phage (so I had to use the TA's soil sample), and my plate titers kept having to get readjusted due to there not being a high enough plate titer.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 6, 2021
Approximate Shotgun Coverage656
Genome length (bp)50645
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGAGCCGTGAT
GC Content67.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBD
SubclusterBD4
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of North Texas
Annotation StatusBeing Annotated (Expected completion by 5/1/2022)
Plaque NotesDiameter: ≤1mm (ranging from pinpoint to small)
More heterogeneous due to diversity in plaque sizes
Slightly more temperate (cloudy) over lytic (clear)
Most plaques have a small clear center and turbid edges, sort of like a bullseye or large halo
Only the smallest plaques are clear
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload