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Streptomyces phage Janus
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Detailed Information for Phage Janus
Discovery Information
Isolation HostStreptomyces xanthochromogenes NRRL B-5410
Found ByJose Molina
Year Found2016
Location FoundDallas, TX USA
Finding InstitutionUniversity of North Texas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates32.730278 N, 96.888611 W Map
Discovery NotesI collected the soil on August 27, 2016 at 7:25 p.m. The temperature at the time was 91 degrees Fahrenheit. I dug about 2 to 3 inches into the ground and collected it; the soil was quite moist and damp.
Naming NotesI decided to name this phage Janus because Janus is the Greek god of beginnings, and I liked it.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 7, 2017
Sequencing FacilityUniversity of North Texas
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage77
Genome length (bp)50632
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGCCGTGTCTT
GC Content67.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBD
SubclusterBD2
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of North Texas
Annotation StatusIn GenBank
Plaque NotesThe average diameter for my phage was about 1mm. It was circular and clear.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK392366
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.2x10^8 pfu/ml
Date of SEA Lysate Titering Nov 10, 2016
Pitt Freezer Box# 36
Pitt Freezer Box Grid# F2
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload