| Detailed Information for Phage Provolone |
| Discovery Information |
| Isolation Host | Streptomyces bicolor NRRL B-3897 |
| Found By | Ioana Rotaru |
| Year Found | 2022 |
| Location Found | Denton, TX United States |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 33.18371 N, 97.10242 W Map |
| Discovery Notes | Soil was collected near a tree at my apartment in southern Denton at the coordinates 33.18371° N, 97.10242° W. I used a small shovel to dig around 1 1/2 in into the ground, then placed the soil inside a ziplock bag. The temperature outside was 79° F, and it was fairly humid and cloudy, however the soil was dry. |
| Naming Notes | This phage was named after a type of cheese I ate on the day of collection. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 11, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1901 |
| Genome length (bp) | 49042 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGGTACGTGAT |
| GC Content | 65.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BD |
| Subcluster | BD3 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of North Texas |
| Annotation Status | Being Annotated (Expected completion by 5/1/2023) |
| Plaque Notes | Clear, circular plaques of around 1mm in diameter. |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4x10^8 |
| Pitt Freezer Box# |
163 |
| Pitt Freezer Box Grid# |
B9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
