Streptomyces phage Provolone
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Detailed Information for Phage Provolone
Discovery Information
Isolation HostStreptomyces bicolor NRRL B-3897
Found ByIoana Rotaru
Year Found2022
Location FoundDenton, TX United States
Finding InstitutionUniversity of North Texas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates33.18371 N, 97.10242 W Map
Discovery NotesSoil was collected near a tree at my apartment in southern Denton at the coordinates 33.18371° N, 97.10242° W. I used a small shovel to dig around 1 1/2 in into the ground, then placed the soil inside a ziplock bag. The temperature outside was 79° F, and it was fairly humid and cloudy, however the soil was dry.
Naming NotesThis phage was named after a type of cheese I ate on the day of collection.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 11, 2023
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1901
Genome length (bp)49042
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGGTACGTGAT
GC Content65.9%
Fasta file available?Yes: Download fasta file
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of North Texas
Annotation StatusBeing Annotated (Expected completion by 5/1/2023)
Plaque NotesClear, circular plaques of around 1mm in diameter.
Has been Phamerated?Yes
Gene List

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Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 4x10^8
Pitt Freezer Box# 163
Pitt Freezer Box Grid# B9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload