Streptomyces phage Thestral
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Detailed Information for Phage Thestral
Discovery Information
Isolation HostStreptomyces xanthochromogenes NRRL B-5410
Found ByMegan Webb
Year Found2016
Location FoundDenton, TX USA
Finding InstitutionUniversity of North Texas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates33.203442 N, 97.15483 W Map
Discovery NotesI collected my soil sample from a pond in Denton. The soil was completed submerged in water with algae present. Animals, mainly ducks, frequent the pond.
Naming NotesI chose the name Thestral after the "death horses" in Harry Potter. I feel the same way for the fictional creatures as I do phages: weirded-out but interested.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedSep 7, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage304
Genome length (bp)52628
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGCCACGTCTT
GC Content67.7%
Sequencing Notesupon assembly 2 cluster BD2's were found in the original sample, they were separated and PCR verified. Mixed phage was named Haizum
Fasta file available?Yes: Download fasta file
Characterization
ClusterBD
SubclusterBD2
Cluster Life CycleTemperate
Lysogeny NotesMy phage is not a temperate phage.
Other Cluster Members
Annotating InstitutionUniversity of North Texas
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Thestral_1

Thestral_2
(HNH endonuclease)

Thestral_4

Thestral_5
(thioredoxin)

Thestral_6
(terminase small subunit)

Thestral_7
(terminase large subunit)

Thestral_8
(portal protein)

Thestral_9

Thestral_10
(scaffolding protein)

Thestral_11
(major capsid protein)

Thestral_12
(head-to-tail connector complex protein)

Thestral_13
(head-to-tail connector complex protein)

Thestral_14

Thestral_15

Thestral_16
(major tail protein)

Thestral_17
(tail assembly chaperone)

Thestral_18
(tail assembly chaperone)

Thestral_19
(tape measure protein)

Thestral_20
(minor tail protein)

Thestral_21
(minor tail protein)

Thestral_22

Thestral_23

Thestral_24

Thestral_25
(minor tail protein)

Thestral_26

Thestral_27
(lysin A)

Thestral_28

Thestral_29

Thestral_30
(immunity repressor)

Thestral_31

Thestral_32
(exonuclease)

Thestral_33

Thestral_34

Thestral_35

Thestral_36
(deoxycytidylate deaminase)

Thestral_37
(DnaB-like dsDNA helicase)

Thestral_38
(holliday junction resolvase)

Thestral_39

Thestral_40
(DNA primase)

Thestral_41
(DNA primase)

Thestral_42

Thestral_43

Thestral_44

Thestral_45
(endonuclease)

Thestral_46

Thestral_47
(helix-turn-helix DNA binding domain protein)

Thestral_48

Thestral_49
(ribonucleotide reductase)

Thestral_50
(methyltransferase)

Thestral_51

Thestral_52

Thestral_53

Thestral_54
(helix-turn-helix DNA binding domain protein)

Thestral_55
(integrase)

Thestral_56

Thestral_57
(thymidylate synthase)

Thestral_58

Thestral_59

Thestral_60

Thestral_61

Thestral_62

Thestral_63

Thestral_64
(deoxynucleoside monophosphate kinase)

Thestral_65

Thestral_66

Thestral_67

Thestral_68

Thestral_69

Thestral_70

Thestral_71

Thestral_72

Thestral_73

Thestral_74

Thestral_75

Thestral_76

Thestral_77

Thestral_78

Thestral_79

Thestral_80

Thestral_81

Thestral_82

Thestral_83

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH651190
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2x10^8 pfu/mL
Pitt Freezer Box# 70
Pitt Freezer Box Grid# G5
Available Files
Plaque PictureDownload
GenBank File for PhameratorDownload