Streptomyces phage Werner
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Detailed Information for Phage Werner
Discovery Information
Isolation HostStreptomyces lividans JI 1326
Found ByNeal Huang and Ethan Cordes
Year Found2019
Location FoundSt. Louis, MO United States
Finding InstitutionWashington University in St. Louis
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature28°C
GPS Coordinates38.6352 N, 90.2687 W Map
Discovery NotesWe named the Werner phage after Werner Heisenberg, specifically because of his famous uncertainty principle. While working with the Werner phage we were always unable to determine whether our solutions actually had any phage particles in them. Without this confirmation, we were always uncertain whether or not our experiments would succeed.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJun 24, 2020
Sequencing FacilityThe McDonnell Genome Institute at Washington University
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage100
Genome length (bp)51566
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGGCCAGTGAT
GC Content65.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBD
SubclusterBD1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionWashington University in St. Louis
Annotation StatusIn GenBank
Plaque NotesThe plaques have a bullseye pattern where the edges are more turbid, and the centers are clearer. The plaques are also ringed in a red pigment and are approximately 3.96mm in diameter. The plaques displayed in the included image were grown on a 100mm plate and were produced using a phage solution diluted to a concentration of 10^5.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Werner_1

Werner_2
(HNH endonuclease)

Werner_3

Werner_4
(thioredoxin)

Werner_5
(terminase small subunit)

Werner_6
(terminase large subunit)

Werner_7
(portal protein)

Werner_8
(capsid maturation protease and MuF-like fusion protein)

Werner_9
(scaffolding protein)

Werner_10
(major capsid protein)

Werner_11
(head-to-tail adaptor)

Werner_12
(head-to-tail stopper)

Werner_13

Werner_14
(tail terminator)

Werner_15
(major tail protein)

Werner_16
(tail assembly chaperone)

Werner_17
(tail assembly chaperone)

Werner_18
(tape measure protein)

Werner_19
(minor tail protein)

Werner_20
(minor tail protein)

Werner_21

Werner_22
(minor tail protein)

Werner_23
(minor tail protein)

Werner_24

Werner_25
(endolysin)

Werner_26

Werner_27

Werner_28
(immunity repressor)

Werner_29

Werner_30
(exonuclease)

Werner_31

Werner_32

Werner_33

Werner_34
(deoxycytidylate deaminase)

Werner_35
(DnaB-like dsDNA helicase)

Werner_36
(holliday junction resolvase)

Werner_37

Werner_38
(DNA primase)

Werner_39
(DNA primase)

Werner_40

Werner_41

Werner_42
(helix-turn-helix DNA binding domain protein)

Werner_43
(MRE11 double-strand break endo/exonuclease)

Werner_44

Werner_45
(helix-turn-helix DNA binding domain protein)

Werner_46

Werner_47
(ribonucleotide reductase)

Werner_48
(methyltransferase)

Werner_49

Werner_50
(serine integrase)

Werner_51
(ThyX-like thymidylate synthase)

Werner_52

Werner_53

Werner_54
(DnaE-like DNA polymerase III alpha)

Werner_55

Werner_56

Werner_57

Werner_58
(deoxynucleoside monophosphate kinase)

Werner_59

Werner_60

Werner_61

Werner_62

Werner_63

Werner_64

Werner_65
(HNH endonuclease)

Werner_66

Werner_67
(OCR-like antirestriction protein)

Werner_68

Werner_69

Werner_70

Werner_71

Werner_72

Werner_73
(acetyltransferase)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMW435856
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload